Animal data onlyNot FDA approved

MOTS-c dosage

Also known as: MOTSc · Mots C · mots · MOTS-c acetate · MOTS-c free base · Mitochondrial open reading frame of the 12S rRNA type-c

Updated Oct 24, 202667 sources

Every MOTS-c protocol on file, 16 in all, labelled by where each number came from. 1 have titration steps, each step is worked out in U-100 units for a 10 mg vial + 0.5 mL water.

Calculate with MOTS-c MOTS-c overview

MOTS-c dosage by goal

Grouped by goal. Each protocol shows the amount, how often, cycle and rest as the source states them, with every titration step.

Injected16

Standard intermittent

Amount: 5 mg
How often: 2 to 3 times a week
Route: SubQ, vial (powder)
Cycle: 4 to 8 weeks
Rest: 4 to 8 weeks off, matched to the run: 4 on and 4 off, 6 on and 4 to 6 off, or 8 on and 6 to 8 off

Note: The most cited starting plan. No human dose is established. FDA found no human exposure data for MOTS-c by any route.

Higher tier

Amount: 10 mg
How often: 2 to 3 times a week
Route: SubQ, vial (powder)
Cycle: 6 to 8 weeks
Rest: 4 to 8 weeks off, matched to the run: 4 on and 4 off, 6 on and 4 to 6 off, or 8 on and 6 to 8 off

Weekly total

Amount: 5 to 10 mg
How often: Weekly total; the guide does not say how to split it
Route: SubQ, vial (powder)

Note: Shown as a weekly amount only. Split across 1 to 3 shots, it lines up with the intermittent plans.

Slow start, then twice weekly

Amount: 5 mg
How often: Once a week for the first 2 weeks, then twice a week
Route: SubQ, vial (powder)
Cycle: 8 to 12 weeks

Note: The guide repeats the cycle 2 to 3 times a year as maintenance.

Performance, pre-training

Amount: 5 mg
How often: Twice a week, 10 mg a week in total
Route: SubQ, vial (powder)
Cycle: 8 to 12 weeks

Timing: 1 to 2 hours before training.

Post-exercise

Amount: 5 mg
How often: Up to 3 times a week, on training days
Route: SubQ, vial (powder)
Cycle: 4 to 8 weeks

Timing: Within 60 minutes after a workout.

20-day, 4-shot cycle

Amount: 5 to 10 mg
How often: Every 5 days, 4 shots in all (days 1, 6, 11 and 16)
Route: SubQ, vial (powder)
Cycle: 20 days, 4 injections
Rest: At least 4 months off, for 2 to 3 cycles a year. Another guide says repeat within 6 months.

Timing: Morning, fasted, before the first meal. One guide says morning because it may interfere with sleep.

Note: Most run 5 mg per shot; some go to 10 mg.

Quick reference range

Amount: 5 to 10 mg
How often: 2 to 3 times a week, or every 5 days
Route: SubQ, vial (powder)
Cycle: 4 to 6 weeks

Timing: Morning or before exercise.

Note: Repeated 2 to 4 times a year.

Daily low-dose ladder (vial math)

Amount: 200 to 1,000 mcg
How often: Once daily, stepping up every 2 weeks
Route: SubQ, vial (powder)

Titration steps for Daily low-dose ladder (vial math)
When	Dose	U-100 units (10 mg vial + 0.5 mL water)
Weeks 1 to 2	200 mcg	1 units · 0.01 mL
Weeks 3 to 4	400 mcg	2 units · 0.02 mL
Weeks 5 to 6	600 mcg	3 units · 0.03 mL
Weeks 7 to 8	800 mcg	4 units · 0.04 mL
Weeks 9 to 10	1,000 mcg	5 units · 0.05 mL

Note: The guide calls this math only, not a recommendation. It sits well below what most users run.

Monthly weekly block

Amount: 10 mg
How often: Once a week for 4 weeks
Route: SubQ, vial (powder)
Cycle: 4 weeks
Rest: The author's rule: no more than 8 weeks on, then 4 off

Daily low dose

Amount: 1 to 2 mg
How often: Once daily
Route: SubQ, vial (powder)

Timing: Morning. Late doses are commonly reported on r/Peptides to wreck sleep.

Note: One writer suggests starting at 0.5 to 1 mg a day and working up over a couple of weeks. The 2 mg report came from a user also on retatrutide who saw low glucose.

Self-titrated every other day

Amount: 1.5 mg
How often: Every other day
Route: SubQ, vial (powder)

Who: One r/Peptides user.

Note: Worked up from 0.5 mg to 1 mg to 2 mg. At 2 mg they were awake until 3 AM, so they settled at 1.5 mg every other day.

Every 3 days

Amount: 5 mg
How often: Every 3 days
Route: SubQ, vial (powder)
Cycle: 10 weeks

Who: One r/Peptides user.

Note: The user said it took about 6 weeks to kick in.

Weekly total split into 3 shots

Amount: 10 mg
How often: 10 mg a week, split into Monday, Wednesday and Friday shots
Route: SubQ, vial (powder)

Timing: Morning.

Note: Shown as a weekly total. Run alongside AOD-9604 by the poster.

Low intermittent

Amount: 0.8 to 1.66 mg
How often: 1 mg twice a week, 0.8 mg every 3 days, or 1.66 mg Monday, Wednesday and Friday
Route: SubQ, vial (powder)
Cycle: 8 weeks for the Monday, Wednesday, Friday plan

Timing: Run after an SS-31 block or alternated with it.

Note: Three separate users from 2026 threads.

Load, then taper

Amount: 5 to 10 mg
How often: 3 times a week for the loading phase, then once a week for 4 weeks
Route: SubQ, vial (powder)
Cycle: 5 mg version: 6 weeks at 3 shots a week, then 4 weeks at 1 shot a week

Note: A 10 mg version runs 3 shots a week, then 10 mg weekly for 4 weeks. Users repeat it 1 to 2 times a year.

Doses shown are what sources report, labelled by origin. Not medical advice.

Community-reported amounts

The MOTS-c amounts people mention most on Reddit, by goal. Counts, not recommendations.

MOTS-c amounts reported on Reddit, by goal, with mention counts
Goal	Most reported amounts (mentions)
Goal not stated	1 mg (51), 5 mg (51), 2 mg (35), 3 mg (23)
Longevity and energy	5 mg (15), 2 mg (10), 1 mg (7), 4 mg (3)
Metabolic health	5 mg (13), 1 mg (9), 3 mg (4), 2 mg (3)

From 900 Reddit posts and comments, last mined Oct 23, 2026.

MOTS-c dosage chart by vial size

Units to draw for the doses sources cite most, at every vial size and water volume.

MOTS-c: concentration and U-100 syringe units for each vial size and water volume
Water	Strength	1,000 mcgdose, units	5 mgdose, units	10 mgdose, units
5 mg vial
0.5 mLVendor guide^†	10 mg/mL	100.1 mL	500.5 mL	1001 mLmore than the vial
1 mLCompetitor guide^†	5 mg/mL	200.2 mL	1001 mL	2002 mL2 draws
2 mLCompetitor guide^†	2.5 mg/mL	400.4 mL	2002 mL2 draws	4004 mL4 draws
3 mLCompetitor guide^†	1.667 mg/mL	600.6 mL	3003 mL3 draws	6006 mL6 draws
10 mg vial
0.5 mLVendor guide	20 mg/mL	50.05 mL	250.25 mL	500.5 mL
1 mLCompetitor guide	10 mg/mL	100.1 mL	500.5 mL	1001 mL
2 mLCompetitor guide	5 mg/mL	200.2 mL	1001 mL	2002 mL2 draws
3 mLCompetitor guide	3.333 mg/mL	300.3 mL	1501.5 mL2 draws	3003 mL3 draws
20 mg vial
2 mLCommunity-reported	10 mg/mL	100.1 mL	500.5 mL	1001 mL
40 mg vial
0.5 mLVendor guide^†	80 mg/mL	1.30.013 mL	6.30.063 mL	12.50.125 mL
1 mLCompetitor guide^†	40 mg/mL	2.50.025 mL	12.50.125 mL	250.25 mL
2 mLCompetitor guide^†	20 mg/mL	50.05 mL	250.25 mL	500.5 mL
3 mLCompetitor guide^†	13.333 mg/mL	7.50.075 mL	37.50.375 mL	750.75 mL

Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.† Water volume sourced for a different vial size. The math is exact either way.Water volumes from

Doses shown are what sources report, labelled by origin. Not medical advice.

Calculate with this peptideYour vial, your water, your dose: MOTS-c in syringe units, with the math shown.

Mixing MOTS-c

Diluent: Bacteriostatic water

Water volumes sources use: 0.5 mL, 1 mL, 2 mL, 3 mL

One guide says 1 to 2 mL per vial depending on dose. 10 mg is the most common vial. Some users report a mix turning milky white overnight, or vials arriving with no vacuum and particles after mixing. Do not share a syringe with another peptide.

If you miss a dose

Dosing guides say skip a missed dose and do not double up. A clinic guide for the every-5-days cycle says inject when you can and count the next 5 days from that shot.

MOTS-c dosage questions

How much MOTS-c should I take?

There is no established human dose. FDA found no human exposure data for MOTS-c by any route. Two camps dominate. Dosing guides favor 5 mg 2 to 3 times a week, or 5 to 10 mg a week in total, run in cycles. A daily camp on r/Peptides runs 0.5 to 2 mg a day. On one tracking site, self-reported shots have a median of 2 to 3 mg, with 5 mg and up the most common bucket.

How often do people inject it?

1 to 3 times a week is the guide standard. A clinic plan uses every 5 days for 4 shots over 20 days. The daily camp injects once a day or every other day.

How much water goes in a 10 mg vial?

1 mL (10 mg per mL) or 2 mL (5 mg per mL) are the most common. At 1 mL, 5 mg is 50 units on a U-100 syringe. Some use 0.5 mL (5 mg is 25 units) or 3 mL. Use the calculator for any other mix.

Why cycle MOTS-c?

Nobody has data on it. Users who go looking find no literature, and one guide calls cycling precautionary. Rest periods in guides range from 4 weeks to 4 months.

How long does mixed MOTS-c last?

In the fridge, claims run from 2 to 7 days (a supplier note quoted by FDA) to 30 days. Most guides say 2 to 4 weeks. Never freeze it. Users ask whether a 40 mg vial can last a month after mixing; nobody has tested research vials.

Why did my mixed vial go cloudy?

Users report MOTS-c turning milky white overnight in the fridge, and some vials arriving with no vacuum and particles after mixing. No authoritative answer exists.

Where do MOTS-c doses come from?

Mostly mice. Studies used 0.5 mg/kg a day by injection into the belly cavity for 8 weeks, 5 mg/kg a day, and 15 mg/kg 3 times a week late in life. Body-surface conversion to a 70 kg adult gives anything from low single-digit milligrams to about 28.5 mg, depending on the guide. These are not human doses.

Sources for these doses

21 sources, each checked by date. Record updated Oct 24, 2026. Numbers are restated in our own words and labelled by where they came from.

Regulatory1

FDA briefing document, PCAC July 23 to 24, 2026: MOTS-cFDA · checked Oct 23, 2026

Vendor guide1

MOTS-c dosage protocol: 4-injection cycleperfectb.com · checked Oct 23, 2026

Competitor guide7

MOTS-c quick start protocolpeptidedosingprotocols.com · checked Oct 23, 2026
MOTS-cpeptiq.io · checked Oct 23, 2026
MOTS-cpeptidesexplorer.com · checked Oct 23, 2026
MOTS-c reportoutliyr.com · checked Oct 23, 2026

Community-reported12

MOTS-C: the mitochondrial peptide nobody explains properlyderekpruski.substack.com · checked Oct 23, 2026
MOTS-c 1 mg a day; mixed vial went milkyr/Peptides · checked Oct 23, 2026
MOTS-c 2 mg a day with retatrutide, low glucose on CGMr/Peptides · checked Oct 23, 2026
MOTS-c as liquid energyr/Peptides · checked Oct 23, 2026

Back to the full MOTS-c page for side effects, storage, stacks and legal status.