Also known as: Epithalon · Epithalone · AEDG · Ala-Glu-Asp-Gly
Updated 83 sources
Every Epitalon protocol on file, 23 in all, labelled by where each number came from.
Note: This guide says no data shows a better result above 10 mg a day. The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day.
Note: The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day.
Note: A 2024 r/Peptides tally of sleep doses: 100 mcg mentioned 8 times, 300 and 500 mcg 4 times each, 250 mcg 3 times. One poster worked back from a monkey study (about 2 mcg/kg) to 100 to 300 mcg. Posters argue synthetic epitalon is more potent than the extract; no study shows a ratio.
Note: Described on r/Peptides as widely accepted for sleep, with 10 mg for 10 days once or twice a year kept for anti-aging. One user started at 200 mcg at bedtime, planning to titrate to at most 1 mg over 10 to 20 days.
Who: Older adults with low pineal function: 15 on epitalon, 15 on epithalamin, 10 on placebo
Note: One of only two published systemic doses of synthetic epitalon in humans. Together they sit roughly 10 to 1,000 times below the community 5 to 10 mg. Night melatonin rose in people who started low.
Who: 40 female night-shift health workers with low melatonin output, 20 on AEDG and 20 on placebo
Note: Dissolved under the tongue. A urinary melatonin marker rose about 1.7-fold. Adverse events were not systematically reported.
Who: 162 people with retinitis pigmentosa in a Russian controlled study
Note: A local eye dose, not a systemic one. No treatment-related complications were described.
Timing: Evening or bedtime, to line up with the nightly melatonin rhythm.
Note: The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day. The guide itself says 1 to 2 courses a year in one place and 2 to 3 in another.
Timing: Evening.
Note: The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day. One guide points out this popular table runs into vial-size problems with 10 mg vials.
Note: The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day.
Note: Close to an epithalamin trial schedule: five 10 mg IM shots, 3 days apart, every 6 months. The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day.
Timing: Evening, 7 to 9 pm.
Note: Guides say run 5 mg on the first course and move to 10 mg on later ones. IM is listed as an alternative at the same dose. The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day.
Note: The same guide calls 20 mg a day not recommended outside specialized research. The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day.
Note: Double the usual daily ceiling. Most guides discourage going past 10 mg a day. The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day.
Note: The only guide protocol without a rest period. The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day.
Note: The 5 to 10 mg figure traces to regimens for epithalamin, the pineal extract, not synthetic epitalon. The human epitalon studies used 0.01 to 0.5 mg a day.
Note: About ten times below the usual guide dose, and the guide does not explain why. It sits close to the 0.5 mg a day used in a human study.
Note: One guide's tier built on the human study dose, kept apart from community convention. The study itself gave 0.5 mg under the tongue, not by injection.
Note: Posted on r/Peptides alongside 100 mcg daily for 10 days twice a year.
Note: Reported runs: 1 mg a night with pinealon for 20 nights, 2 mg for 20 days, 2.5 mg each morning for 12 days. One user felt nothing below 1 mg, then slept hard with wild dreams at 3 mg.
Note: Commonly reported on r/Peptides. Some go higher: 10 to 15 mg for 20 days, or 20 mg a night. Top replies in a long thread say the 10 mg for 10 days regimen was written for epithalamin, the extract. Others answer that nobody has shown synthetic epitalon is stronger.
Note: Sold as 3.3 mg capsules, 30 a bottle. No study has measured how much survives digestion.
Doses shown are what sources report, labelled by origin. Not medical advice.
| Goal | Most reported amounts (mentions) |
|---|---|
| Goal not stated | 10 mg (49), 500 mcg (26), 1 mg (23), 5 mg (20) |
| Sleep | 500 mcg (12), 1 mg (9), 100 mcg (7), 5 mg (3) |
| Longevity and energy | 10 mg (6), 5 to 10 mg (5), 1 mg (4), 100 mcg (3) |
| Water | Strength | 0.5 mgdose, units | 5 mgdose, units | 10 mgdose, units |
|---|---|---|---|---|
| 10 mg vial | ||||
| 1 mLCompetitor guide | 10 mg/mL | 50.05 mL | 500.5 mL | 1001 mL |
| 2 mLCompetitor guide | 5 mg/mL | 100.1 mL | 1001 mL | 2002 mL2 draws |
| 50 mg vial | ||||
| 2 mLCompetitor guide | 25 mg/mL | 20.02 mL | 200.2 mL | 400.4 mL |
| 5 mLCompetitor guide | 10 mg/mL | 50.05 mL | 500.5 mL | 1001 mL |
| 100 mg vial | ||||
| 1 mLCompetitor guide† | 100 mg/mL | 0.50.005 mLunder 1 unit | 50.05 mL | 100.1 mL |
| 2 mLCompetitor guide† | 50 mg/mL | 10.01 mL | 100.1 mL | 200.2 mL |
| 5 mLCompetitor guide† | 20 mg/mL | 2.50.025 mL | 250.25 mL | 500.5 mL |
Doses shown are what sources report, labelled by origin. Not medical advice.
Calculate with this peptideYour vial, your water, your dose: Epitalon in syringe units, with the math shown.Diluent: Bacteriostatic water
Water volumes sources use: 1 mL, 2 mL, 5 mL
2 mL in a 10 mg vial (5 mg/mL) is the most common mix. At that strength a 5 mg dose fills a whole 1 mL syringe and a 10 mg vial lasts 2 days, so a 20-day course at 5 mg a day needs about 10 vials and 20 mL of water. Some posters mix stronger, such as 20 mg/mL, so bigger doses fit a small syringe.
It depends on whose numbers you use. Most guides say 5 to 10 mg a day by injection for 10 to 20 days. Sleep users on r/Peptides mostly run 100 to 500 mcg. The two human studies of synthetic epitalon used 0.01 mg a day by IM injection and 0.5 mg a day under the tongue. No human dose-finding study exists.
It appears to come from epithalamin, the bovine pineal extract epitalon was modeled on. Reviews of the Russian trials give epithalamin IM at 5 to 10 mg a day for 10 to 20 days, and one 3-year trial used 10 mg IM shots. The only published systemic doses of synthetic epitalon in humans are 0.01 mg IM and 0.5 mg under the tongue, roughly 10 to 1,000 times lower. Whether the extract figure transfers to the pure peptide has never been tested.
No, the 10 mg figure is real. A Russian speaker on r/Peptides posted the original paper showing 10 mg a day for 10 days by IM. The top replies point out that regimen was for epithalamin, the extract, not epitalon. A 2026 review agrees the human epitalon studies used far lower doses.
10 to 20 days is the convention. Shorter courses use higher daily doses, such as 10 mg for 10 days against 5 mg for 20.
Guides say 1 to 3 times a year, with 3 to 6 or 4 to 6 months between courses. Some guides contradict themselves on this within the same page.
2 mL is most common and makes 5 mg/mL, so 5 mg is 100 units on a U-100 syringe. 1 mL makes 10 mg/mL. For a 50 mg vial, guides use 5 mL (10 mg/mL) or 2 mL (25 mg/mL). Use the calculator for any other mix.
Guides range from 1 to 2 weeks up to 30 days in the fridge. None has stability data. Keep it at 2 to 8 C, never freeze it, and toss it if it turns cloudy.
40 sources, each checked by date. Record updated Oct 23, 2026. Numbers are restated in our own words and labelled by where they came from.
Back to the full Epitalon page for side effects, storage, stacks and legal status.