Short senolytic pulse
Note: The pattern most guides describe: copy the mouse schedule of 3 doses on alternate days, then stop for months. 2 mg is the most cited amount. No human trial backs any of these doses.
Animal data onlyNot FDA approved
FOXO4-DRI dosage
Also known as: FOXO4 DRI · FOXO4-D-Retro-Inverso · FOXO4 peptide · FOX04-DRI
Updated 26 sources
Every FOXO4-DRI protocol on file, 7 in all, labelled by where each number came from. 1 have titration steps, each step is worked out in U-100 units for a 10 mg vial + 2 mL water.
FOXO4-DRI dosage by goal
Grouped by goal. Each protocol shows the amount, how often, cycle and rest as the source states them, with every titration step.
Injected7
First course, low dose
Note: Described as a conservative approach for a first run.
Extended alternate-day course
Note: One guide lists 2 mg every other day for 5 doses (10 mg per course), run once a year. Another reports people stepping up to 3 to 8 mg per dose over several courses.
Higher-dose pulse
Note: The top of this range comes from scaling the mouse dose to an adult; one guide puts the total per course at 6 to 75 mg.
Three days in a row
Note: This guide suggests starting at 1 mg a day for 3 days on a first run. Another guide's community table lists 6 to 8 mg daily for 3 to 4 days for experienced users.
Five nightly doses (one blogger)
Timing: Before bed
Note: A single person's published log, not a protocol anyone tested. She reported headaches, fatigue and a gut flare during the course.
Daily low-dose ladder (outlier)
Timing: Any consistent time
| When | Dose | U-100 units (10 mg vial + 2 mL water) |
|---|---|---|
| Weeks 1 to 4 | 250 mcg | 5 units · 0.05 mL |
| Weeks 5 to 8 | 375 mcg | 7.5 units · 0.075 mL |
| Weeks 9 to 16 | 500 mcg | 10 units · 0.1 mL |
Note: Two template-style guides list this daily ladder. It runs against both the mouse study and community practice, which use a few large doses and then stop. Neither guide gives a reason for daily use.
Doses shown are what sources report, labelled by origin. Not medical advice.
FOXO4-DRI dosage chart by vial size
Units to draw for the doses sources cite most, at every vial size and water volume.
| Water | Strength | 1 mgdose, units | 2 mgdose, units | 3 mgdose, units |
|---|---|---|---|---|
| 10 mg vial | ||||
| 1 mLCompetitor guide | 10 mg/mL | 100.1 mL | 200.2 mL | 300.3 mL |
| 2 mLCompetitor guide | 5 mg/mL | 200.2 mL | 400.4 mL | 600.6 mL |
| 3 mLCompetitor guide | 3.333 mg/mL | 300.3 mL | 600.6 mL | 900.9 mL |
Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.Water volumes from
Doses shown are what sources report, labelled by origin. Not medical advice.
Calculate with this peptideYour vial, your water, your dose: FOXO4-DRI in syringe units, with the math shown.Mixing FOXO4-DRI
Diluent: Bacteriostatic water
Water volumes sources use: 1 mL, 2 mL, 3 mL
2 mL suits the 1 to 2 mg community pulses; 3 mL suits the small daily ladder. One guide says saline may cause precipitation. The solution should be clear; discard it if cloudy.
FOXO4-DRI dosage questions
What is the FOXO4-DRI dose?
No human dose exists. Community courses copy the mouse schedule: 3 doses every other day, most often 2 mg each, with reported ranges from 1 mg to 10 mg and some guides listing up to 25 mg. Then people stop for months.
How was FOXO4-DRI dosed in the mouse study?
In Baar et al. 2017: 5 mg per kg, 3 doses every other day (days 1, 3 and 5), into a vein for the chemotherapy (doxorubicin) mice and into the abdomen for naturally aged mice. The aged mice were assessed 30 days after the injections. It is the only published whole-animal regimen, it was never tested in people, and mouse doses do not carry over to humans by body weight.
Why not just scale the mouse dose to a human?
Guides that convert by body surface area get roughly 25 mg per dose for a 60 kg adult or about 28.5 mg for 70 kg, far above what most users inject. One guide warns the conversion carries large uncertainty, and no human study has checked any of it.
How often can you repeat a FOXO4-DRI course?
Guides say 1 to 3 courses a year, with at least 4 to 6 months between them as the community consensus.
How much bacteriostatic water for a 10 mg vial?
Guides use 1, 2 or 3 mL. 2 mL gives 5 mg per mL, so a 2 mg dose is 40 units; 3 mL suits small daily doses.
How many units is 2 mg of FOXO4-DRI?
40 units on a U-100 insulin syringe when a 10 mg vial is mixed with 2 mL. With 1 mL it is 20 units.
How long does mixed FOXO4-DRI last?
Guides say about 28 days in the fridge at 2 to 8 C. Since a course is only 3 to 5 doses, most people finish a vial well inside that.
Sources for these doses
11 sources, each checked by date. Record updated Oct 24, 2026. Numbers are restated in our own words and labelled by where they came from.
Vendor guide1
- FOXO4-DRI (10mg) product listingVendor product page · checked Oct 24, 2026
Competitor guide4
- FOXO4-DRI peptide: benefits, dosage and side effectspeptidedeck.com · checked Oct 24, 2026
- FOXO4-DRI 10 mg vial dosage protocolpeptidedosages.com · checked Oct 24, 2026
- FOXO4-DRI quick start protocolpeptidedosingprotocols.com · checked Oct 24, 2026
- FOXO4-DRI 10 mg dosing calculatorpeptideprotocolwiki.com · checked Oct 24, 2026
Community-reported6
- FOXO4-DRI community protocols and anecdotal reportspeptideprotocolwiki.com, via a competitor guide · checked Oct 24, 2026
- FOXO4-DRI: what the research actually showspeptibase.dev, via a competitor guide · checked Oct 24, 2026
- FOXO4-DRI: the senolytic peptide that restored old mice in one studypeptidebreakdown.com, via a competitor guide · checked Oct 24, 2026
- FOXO4-DRI dosage guide: senolytic protocolspeptidewiki.co, via a competitor guide · checked Oct 24, 2026
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