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Animal data onlyNot FDA approved

HGH Fragment 176-191 dosage

Also known as: HGH Frag 176-191 · HGH Frag · hGH 176-191 · Fragment 176-191 · Frag 176-191 · Frag 176 · GH fragment 176-191

Updated 33 sources

Every HGH Fragment 176-191 protocol on file, 7 in all, labelled by where each number came from.

HGH Fragment 176-191 dosage by goal

Grouped by goal. Each protocol shows the amount, how often, cycle and rest as the source states them, with every titration step.

Fat loss2

Fat loss, common community range

Amount
250 to 500 mcg
How often
250 to 500 mcg a day, as one or two fasted injections
Route
SubQ, vial (powder)
Cycle
8 to 12 weeks

Who: Adults using research peptides; no human trial has dosed the unmodified fragment.

Timing: Fasted, usually first thing in the morning; wait 30 to 60 minutes before eating. A second dose goes at least 3 hours after the last meal.

Note: The range is community practice. The rodent studies behind the fragment used far higher doses per kg, so this is not a scaled trial dose.

Fat loss, guide protocol with breaks

Amount
250 to 500 mcg
How often
250 to 500 mcg per injection, once or twice daily
Route
SubQ, vial (powder)
Cycle
4 to 8 weeks on
Rest
2 to 4 weeks off

Timing: Morning fasted and/or before bed on an empty stomach; 30 minutes before meals.

Note: The guide calls this a research protocol and also says no official human dosing exists.

Other protocols: injected5

Beginner start

Amount
250 mcg
How often
Once daily, morning fasted
Route
SubQ, vial (powder)
Cycle
6 to 8 weeks

Timing: Morning on an empty stomach; wait at least 30 minutes before eating.

Split dose, morning and bedtime

Amount
250 mcg
How often
Twice daily: on waking fasted and before bed
Route
SubQ, vial (powder)
Cycle
8 to 12 weeks

Timing: Both doses on an empty stomach; the evening dose at least 3 hours after the last meal.

Note: The guide calls this the most widely used schedule.

Advanced, higher split dose

Amount
500 mcg
How often
Twice daily: morning fasted and before bed
Route
SubQ, vial (powder)
Cycle
12 to 16 weeks
Rest
4 weeks off

Note: One guide's top tier, for people who already ran the standard schedule.

Higher single morning dose

Amount
500 to 1,000 mcg
How often
Once daily, morning fasted
Route
SubQ, vial (powder)
Cycle
4 to 8 weeks

Note: The guide cites "clinical trial data showing 1 mg effective dose" without naming a trial; no human trial has dosed this fragment, and the 1 mg AOD-9604 results FDA summarizes are for a different molecule. One Reddit user ran 500 to 1000 mcg every morning for 2 months and reported no result.

Before fasted cardio or training

Amount
250 to 500 mcg
How often
5 days a week: 250 mcg before fasted morning cardio plus 250 mcg before training, or 500 mcg before training
Route
SubQ, vial (powder)

Timing: Fasted, just before cardio or a workout.

Note: No study has tested pre-cardio timing; one guide calls it plausible folklore.

Doses shown are what sources report, labelled by origin. Not medical advice.

HGH Fragment 176-191 dosage chart by vial size

Units to draw for the doses sources cite most, at every vial size and water volume.
HGH Fragment 176-191: concentration and U-100 syringe units for each vial size and water volume
WaterStrength250 mcgdose, units500 mcgdose, units1,000 mcgdose, units
2 mg vial
0.8 mLCompetitor guide2.5 mg/mL100.1 mL200.2 mL400.4 mL
1 mLCompetitor guide2 mg/mL12.50.125 mL250.25 mL500.5 mL
2 mLCompetitor guide1 mg/mL250.25 mL500.5 mL1001 mL
5 mg vial
2 mLCompetitor guide2.5 mg/mL100.1 mL200.2 mL400.4 mL
2.5 mLCompetitor guide2 mg/mL12.50.125 mL250.25 mL500.5 mL
10 mg vial
2 mLCompetitor guide5 mg/mL50.05 mL100.1 mL200.2 mL
Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.Water volumes from

Doses shown are what sources report, labelled by origin. Not medical advice.

Calculate with this peptideYour vial, your water, your dose: HGH Fragment 176-191 in syringe units, with the math shown.

Mixing HGH Fragment 176-191

Diluent: Bacteriostatic water; some users add a little acetic acid first

Water volumes sources use: 0.8 mL, 1 mL, 2 mL, 2.5 mL

Gelling and cloudiness are common complaints, including 5 mg vials that turned to gel after 1 mL of BAC water. The usual community fix is a small amount of acetic acid first, then BAC water; reported strengths vary widely (0.6% in one post, 30% in another). Pick one water volume and keep it the same for every vial.

HGH Fragment 176-191 dosage questions

What is the usual HGH Frag 176-191 dose?

Community practice is 250 to 500 mcg a day, injected under the skin fasted, as one or two doses, for 8 to 12 weeks. No human trial set this dose.

How many units is 250 mcg of HGH Frag?

It depends on the water. A 5 mg vial with 2 mL of bacteriostatic water puts 250 mcg at 10 units on a U-100 insulin syringe. A 2 mg vial with 2 mL puts it at 25 units. Use the calculator for your own vial.

How long does reconstituted HGH Frag last?

Guides say refrigerate at 2 to 8 C and use it within 14 to 21 days; one says 28 days. Do not freeze it once mixed.

Sources for these doses

12 sources, each checked by date. Record updated Oct 24, 2026. Numbers are restated in our own words and labelled by where they came from.

Vendor guide1

  1. HGH Fragment (176-191), catalog LT183171LifeTein (vendor product page) · checked Oct 24, 2026

Competitor guide3

  1. HGH Fragment 176-191 Dosing Protocols and Administrationpeptideprotocolwiki.com · checked Oct 24, 2026
  2. HGH Fragment 176-191: Dosage, Benefits & Safetypeptidesexplorer.com · checked Oct 24, 2026
  3. HGH Fragment 176-191 10 mg dosing calculatorpeptideprotocolwiki.com · checked Oct 24, 2026

Community-reported7

  1. HGH Fragment 176-191 Dosage: What Research Supportspeptidesexplorer.com, via a competitor guide · checked Oct 24, 2026
  2. Comment: frag/CJC/ipamorelin blend math (r/BodyHackGuide, 2026-03-25)Reddit · checked Oct 24, 2026
  3. HGH Fragment 176-191 2 mg dosing calculatorpeptideprotocolwiki.com, via a competitor guide · checked Oct 24, 2026
  4. Comment: daily stack with HGH Frag morning and before bed (r/Peptides, 2026-07-01)Reddit · checked Oct 24, 2026

Back to the full HGH Fragment 176-191 page for side effects, storage, stacks and legal status.