Animal data onlyNot FDA approved

Humanin dosage

Also known as: HN · HNG · S14G-humanin · [Gly14]-humanin · Humanin-G · Humanin peptide

Updated Oct 24, 202625 sources

Every Humanin protocol on file, 10 in all, labelled by where each number came from.

Humanin dosage by goal

Grouped by goal. Each protocol shows the amount, how often, cycle and rest as the source states them, with every titration step.

Native humanin2

Native humanin, standard

Amount: 1 to 3 mg
How often: 2 to 3 times a week
Route: SubQ, vial (powder)
Cycle: 8 to 12 weeks

Note: For the native 24 amino acid peptide, not HNG. No validated human dose exists.

Native humanin, conservative

Amount: 1 mg
How often: Twice a week
Route: SubQ, vial (powder)
Cycle: 8 to 12 weeks
Rest: 4 to 6 weeks off

HNG3

HNG, low microgram dose

Amount: 100 to 500 mcg
How often: 2 to 3 times a week
Route: SubQ, vial (powder)
Cycle: 8 to 12 weeks

Note: HNG is reported as about 1000 times more potent than native humanin in lab tests, so this guide doses it far lower by mass. Check which molecule the vial holds before using any number here.

HNG, community standard

Amount: 1 to 5 mg
How often: 2 to 3 times a week
Route: SubQ, vial (powder)
Cycle: 4 to 8 weeks on
Rest: 2 to 4 weeks off

Note: The most common pattern in one community roundup, which says most users prefer HNG. The same roundup lists a niche plan of 5 to 10 mg total a week split into 2 to 3 shots, 2 to 4 weeks on and off.

HNG, daily

Amount: 0.5 to 2 mg
How often: Once daily
Route: SubQ, vial (powder)
Cycle: 8 to 12 weeks on
Rest: 4 to 8 weeks off

Timing: Morning

Note: Users start at 0.5 mg a day in week 1; reports cluster around 1 mg a day.

Other protocols: injected5

Low daily dose

Amount: 0.5 to 1 mg
How often: Once daily
Route: SubQ, vial (powder)
Cycle: 4 to 8 weeks

Weekly dose

Amount: 0.5 to 2 mg
How often: Once a week (most common), or 0.5 mg daily
Route: SubQ, vial (powder)
Cycle: 4 to 8 weeks
Rest: 2 to 4 weeks off, 2 to 4 cycles a year

Higher daily range

Amount: 1 to 5 mg
How often: Once daily
Route: SubQ, vial (powder)
Cycle: 4 to 8 weeks

Note: Reported range only; the guide says no correct dose exists because no human trial set one.

Every 4 to 5 days (Reddit)

Amount: 5 mg
How often: Once every 4 to 5 days
Route: SubQ, vial (powder)

Note: From a Reddit discussion, read second-hand. Other posts in the same roundup report 2 mg every other day.

HNG microdose (Reddit)

Amount: 25 to 50 mcg
How often: Once daily
Route: SubQ, vial (powder)

Note: One Reddit user's claim, read second-hand; the roundup says the figure is for HNG, not native humanin.

Doses shown are what sources report, labelled by origin. Not medical advice.

Humanin dosage chart by vial size

Units to draw for the doses sources cite most, at every vial size and water volume.

Humanin: concentration and U-100 syringe units for each vial size and water volume
Water	Strength	500 mcgdose, units	1 mgdose, units	5 mgdose, units
5 mg vial
1 mLCompetitor guide	5 mg/mL	100.1 mL	200.2 mL	1001 mL
2 mLCompetitor guide	2.5 mg/mL	200.2 mL	400.4 mL	2002 mL2 draws
10 mg vial
1 mLCompetitor guide	10 mg/mL	50.05 mL	100.1 mL	500.5 mL
2 mLCompetitor guide	5 mg/mL	100.1 mL	200.2 mL	1001 mL
15 mg vial
1 mLCompetitor guide^†	15 mg/mL	3.30.033 mL	6.70.067 mL	33.30.333 mL
2 mLCompetitor guide^†	7.5 mg/mL	6.70.067 mL	13.30.133 mL	66.70.667 mL
20 mg vial
2 mLCompetitor guide	10 mg/mL	50.05 mL	100.1 mL	500.5 mL

Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.† Water volume sourced for a different vial size. The math is exact either way.Water volumes from

Doses shown are what sources report, labelled by origin. Not medical advice.

Calculate with this peptideYour vial, your water, your dose: Humanin in syringe units, with the math shown.

Mixing Humanin

Diluent: Bacteriostatic water

Water volumes sources use: 1 mL, 2 mL

Native humanin and HNG are often sold under the same name. The mass on the label means very different strength depending on which one it is.

Humanin dosage questions

What is the humanin dose?

No human dose has been tested. Guides list native humanin at 1 to 3 mg 2 to 3 times a week, and HNG anywhere from 100 mcg to 5 mg, weekly to daily. Community reports run from 25 mcg a day to 5 mg every few days.

How was humanin dosed in mice?

Only mice have been dosed, always with the HNG analog. Yen et al. 2020 gave 4 mg per kg twice a week into the abdomen for 14 months, starting at 18 months of age; metabolic markers improved and inflammation and IGF-1 fell, with no lifespan difference. Zhang et al. 2012 gave 5 mg per kg a day into the abdomen for 3 months to 9-month-old Alzheimer model mice; memory improved and amyloid plaque fell (the 5 mg per kg figure is read from a guide's summary, since the abstract gives no dose). None of these is a human dose.

How many units is 1 mg of humanin?

20 units on a U-100 insulin syringe with a 10 mg vial and 2 mL of water. With 1 mL of water it is 10 units.

Sources for these doses

8 sources, each checked by date. Record updated Oct 24, 2026. Numbers are restated in our own words and labelled by where they came from.

Competitor guide3

Humanin peptide: what the 2026 research actually showspeptidedeck.com · checked Oct 24, 2026
Humanin dosing calculatorpeptideprotocolwiki.com · checked Oct 24, 2026
Humanin quick startpeptidedosingprotocols.com · checked Oct 24, 2026

Community-reported5

Humanin reportoutliyr.com, via a competitor guide · checked Oct 24, 2026
Humanin: community protocols and reportspeptideprotocolwiki.com, via a competitor guide · checked Oct 24, 2026
Humanin dosage chart: HNG daily protocolthepeptidecatalog.com, via a competitor guide · checked Oct 24, 2026
Humanin peptide: benefits, dosing, safetypeptidesexplorer.com, via a competitor guide · checked Oct 24, 2026

Back to the full Humanin page for side effects, storage, stacks and legal status.