Limited human dataFDA 503A Category 1

NAD+ dosage

Also known as: NAD · NAD plus · Nicotinamide adenine dinucleotide · beta-NAD · NAD peptide · NAD 500 · NAD 1000

Updated Oct 23, 202660 sources

Every NAD+ protocol on file, 19 in all, labelled by where each number came from.

NAD+ dosage by goal

Grouped by goal. Each protocol shows the amount, how often, cycle and rest as the source states them, with every titration step.

Starter2

Starter (subQ)

Amount: 25 mg
How often: Twice a week
Route: SubQ, vial (powder)
Cycle: 2 weeks, then move to the standard plan

Timing: Morning. Later doses keep some people awake.

Note: No controlled human study has set a subQ dose. This is a guide convention for testing tolerance.

Starter (subQ, community)

Amount: 25 to 50 mg
How often: Twice a week
Route: SubQ, vial (powder)

Note: Posters on r/Peptides who had a bad first reaction restart at 15 to 25 mg and build up.

IV study dose2

IV study dose, 6 days

Amount: 1,000 mg
How often: Once daily for 6 days
Route: Intravenous (IV)
Cycle: 6 days

Who: Study summarized in a 2026 review (Braidy 2018)

IV study dose, 4 days

Amount: 500 mg
How often: Once daily for 4 consecutive days, in 500 mL saline
Route: Intravenous (IV)
Cycle: 4 days

Who: 6 clients given NAD+ IV, compared with NR IV (Reyna 2026)

Note: Every NAD+ client had moderate to severe infusion symptoms that ended when the drip ended. Mean infusion time was 97 minutes against 37 for NR.

Nasal spray2

Nasal spray

Amount: 10 mg
How often: One 5 mg spray per nostril, once or twice a day (10 to 20 mg a day)
Route: Nasal, nasal spray

Note: Based on a 500 mg in 10 mL spray (50 mg/mL, 5 mg per 0.1 mL spray). No human study has measured blood NAD+ after a nasal dose; the only recent intranasal paper is in mice.

Nasal spray, higher dose

Amount: 100 mg
How often: Once daily, one spray
Route: Nasal, nasal spray

Note: A sample schedule from one guide. Another lists 50 to 200 mg by nasal spray or under the tongue without a schedule. No human data on either route.

Other protocols: injected8

Standard (subQ, where most home plans land)

Amount: 50 mg
How often: 3 times a week
Route: SubQ, vial (powder)
Cycle: 8 to 12 weeks, then a break

Timing: Morning.

Note: Keep each shot at 1 mL or less per site and split bigger doses. No human subQ NAD+ study has been published.

Standard range (subQ)

Amount: 50 to 100 mg
How often: 2 to 3 times a week (one guide allows up to 5)
Route: SubQ, vial (powder)

Timing: Morning. Push slowly, over 5 to 10 seconds or longer.

Note: Starts with a 50 mg assessment dose, then rises about 25 mg at a time (50, 75, 100 mg) if tolerated. Keep the weekly total near 300 mg without further evaluation. This guide says NAD+ does not have to be cycled.

Daily ramp over 16 weeks (subQ)

Amount: 50 to 100 mg
How often: Once daily, stepping 50 then 75 then 100 mg
Route: SubQ, vial (powder)
Cycle: 16 weeks

Note: The guide does not say how many weeks each step lasts. It also notes there is no published human dose for daily injection.

Higher end (subQ)

Amount: 100 to 250 mg
How often: Daily, or 3 to 5 times a week
Route: SubQ, vial (powder)

Timing: Morning.

Note: Weekly totals here run far past the roughly 300 mg a week ceiling other guides use.

Loading phase (subQ)

Amount: 250 to 500 mg
How often: 500 mg daily for 5 days, then 250 mg 3 times a week
Route: SubQ, vial (powder)

Timing: Morning.

Note: One guide's loading scheme and the top of the published home range. Guides cap each shot at 1 mL per site, so big doses get split across sites.

Common community dose (subQ)

Amount: 50 to 100 mg
How often: 3 to 4 days a week
Route: SubQ, vial (powder)

Timing: Morning is near universal.

Note: Commonly reported on r/Peptides, often as 100 mg a shot. One 3-year user settled at 60 to 75 mg 3 to 4 times a week after running 100 mg. Often run alongside a GLP-1 for daytime energy.

Higher community doses (subQ)

Amount: 50 to 200 mg
How often: 3 times a week
Route: SubQ, vial (powder)

Note: A range r/Peptides posters say they have seen. One user with chronic fatigue reported 150 mg daily, injecting about 30 mg, pausing 1 to 2 minutes, then finishing over 3 to 5 minutes.

Intramuscular

Amount: 50 to 100 mg
How often: 1 to 3 times a week
Route: IM, vial (powder)

Note: A registered trial is testing 100 mg NAD+ in 2 mL bacteriostatic water by IM, subQ and IV push against nicotinamide riboside and placebo. No results are posted yet.

Other protocols: intravenous (iv)5

IV maintenance (clinic)

Amount: 250 to 1,000 mg
How often: Weekly or every other week
Route: Intravenous (IV)

Timing: Infused over about 2 to 4 hours per session.

Note: Most sessions are 250 to 500 mg, with some clinics going to 500 to 1,000 mg. Start the drip at 1 to 2 mg per minute and speed up only as tolerated. Mixed IV bags are used within 24 hours.

IV weekly series (clinic)

Amount: 250 mg
How often: Weekly for 4 weeks, then monthly
Route: Intravenous (IV)
Cycle: 4 weekly infusions, then monthly maintenance

IV loading series (clinic)

Amount: 500 to 1,000 mg
How often: 2 to 3 times a week for 2 to 4 weeks, then monthly
Route: Intravenous (IV)
Cycle: 2 to 4 weeks, then monthly

Note: One guide uses 500 mg 2 to 3 times a week for 2 weeks. Another describes 8 infusions of 1,000 mg across 4 weeks.

IV intensive (addiction and neuro programs)

Amount: 500 to 1,000 mg
How often: Daily for 7 to 10 days
Route: Intravenous (IV)
Cycle: 7 to 10 days

Timing: Each infusion runs 4 to 8 hours. Start at 1 to 2 mg per minute.

Note: Multi-day IV programs for withdrawal and neurodegenerative use are offered by clinics and pharmacies. No controlled trial backs this use.

IV pilot study dose

Amount: 750 mg
How often: One infusion over 6 hours (about 2 mg per minute)
Route: Intravenous (IV)
Cycle: Single infusion

Who: 8 healthy men on NAD+ and 3 on saline (Grant 2019)

Note: Plasma NAD+ did not rise for the first 2 hours, as if cleared as fast as it went in, then rose 398% by 6 hours. No adverse events were reported.

Doses shown are what sources report, labelled by origin. Not medical advice.

Community-reported amounts

The NAD+ amounts people mention most on Reddit, by goal. Counts, not recommendations.

NAD+ amounts reported on Reddit, by goal, with mention counts
Goal	Most reported amounts (mentions)
Goal not stated	100 mg (55), 50 mg (42), 25 mg (32), 500 mg (20)
Longevity and energy	100 mg (17), 50 mg (12), 25 mg (10), 20 mg (4)
Focus and mood	10 mg (2)

From 900 Reddit posts and comments, last mined Oct 23, 2026.

NAD+ dosage chart by vial size

Units to draw for the doses sources cite most, at every vial size and water volume.

NAD+: concentration and U-100 syringe units for each vial size and water volume
Water	Strength	25 mgdose, units	50 mgdose, units	100 mgdose, units
100 mg vial
2.5 mLCompetitor guide^†	40 mg/mL	62.50.625 mL	1251.25 mL2 draws	2502.5 mL3 draws
3 mLCompetitor guide^†	33.333 mg/mL	750.75 mL	1501.5 mL2 draws	3003 mL3 draws
5 mLCompetitor guide^†	20 mg/mL	1251.25 mL2 draws	2502.5 mL3 draws	5005 mL5 draws
7.5 mLCompetitor guide^†	13.333 mg/mL	187.51.875 mL2 draws	3753.75 mL4 draws	7507.5 mL8 draws
10 mLCompetitor guide^†	10 mg/mL	2502.5 mL3 draws	5005 mL5 draws	100010 mL10 draws
250 mg vial
2.5 mLCompetitor guide^†	100 mg/mL	250.25 mL	500.5 mL	1001 mL
3 mLCompetitor guide^†	83.333 mg/mL	300.3 mL	600.6 mL	1201.2 mL2 draws
5 mLCompetitor guide^†	50 mg/mL	500.5 mL	1001 mL	2002 mL2 draws
7.5 mLCompetitor guide^†	33.333 mg/mL	750.75 mL	1501.5 mL2 draws	3003 mL3 draws
10 mLCompetitor guide^†	25 mg/mL	1001 mL	2002 mL2 draws	4004 mL4 draws
500 mg vial
2.5 mLCompetitor guide	200 mg/mL	12.50.125 mL	250.25 mL	500.5 mL
3 mLCompetitor guide	166.667 mg/mL	150.15 mL	300.3 mL	600.6 mL
5 mLCompetitor guide	100 mg/mL	250.25 mL	500.5 mL	1001 mL
750 mg vial
7.5 mLCompetitor guide	100 mg/mL	250.25 mL	500.5 mL	1001 mL
1,000 mg vial
3 mLCompetitor guide	333.333 mg/mL	7.50.075 mL	150.15 mL	300.3 mL
5 mLCompetitor guide	200 mg/mL	12.50.125 mL	250.25 mL	500.5 mL
10 mLCompetitor guide	100 mg/mL	250.25 mL	500.5 mL	1001 mL
1,500 mg vial
2.5 mLCompetitor guide^†	600 mg/mL	4.20.042 mL	8.30.083 mL	16.70.167 mL
3 mLCompetitor guide^†	500 mg/mL	50.05 mL	100.1 mL	200.2 mL
5 mLCompetitor guide^†	300 mg/mL	8.30.083 mL	16.70.167 mL	33.30.333 mL
7.5 mLCompetitor guide^†	200 mg/mL	12.50.125 mL	250.25 mL	500.5 mL
10 mLCompetitor guide^†	150 mg/mL	16.70.167 mL	33.30.333 mL	66.70.667 mL

Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.† Water volume sourced for a different vial size. The math is exact either way.Water volumes from

Doses shown are what sources report, labelled by origin. Not medical advice.

Calculate with this peptideYour vial, your water, your dose: NAD+ in syringe units, with the math shown.

Mixing NAD+

Diluent: Bacteriostatic water

Water volumes sources use: 2.5 mL, 3 mL, 5 mL, 7.5 mL, 10 mL

The most repeated mix is 500 mg with 5 mL, which makes 100 mg/mL so 1 unit on a U-100 syringe is 1 mg. Posters use 3 to 5 mL for 500 mg and say more water stings less. Strong mixes crystallize in the fridge: one user got crystals at 200 mg/mL, and replies blame anything above about 250 mg/mL when cold. Use more water.

NAD+ dosage questions

How much bacteriostatic water for a 500 mg NAD+ vial?

Most guides say 5 mL. That makes 100 mg/mL, so 1 unit on a U-100 insulin syringe is 1 mg and a 50 mg dose is 50 units. Some guides use 3 mL, which makes about 167 mg/mL and gets close to the strength where crystals become a problem in the fridge. Use the calculator for any other mix.

How much NAD+ should I inject?

Most home subQ use sits at 25 to 100 mg a shot. Guides start around 25 mg twice a week, and 50 mg 3 times a week is where most home plans land. Raise about 25 mg at a time if it goes well. No human study has set a subQ dose, so all of this is convention.

How often do you take NAD+ injections?

Two to three times a week is the norm. Some guides go daily, at 100 to 250 mg, and one guide runs a 16-week daily ramp. Weekly totals across guides range from 50 mg to 1,750 mg, which shows how loose the convention is.

How long does mixed NAD+ last?

About 4 weeks in the fridge by convention. Guides range from 7 to 30 days, and none has stability data. Toss it if it turns yellow or brown, cloudy or grainy.

Is there a weekly limit for subQ NAD+?

Pharmacy guidance starts around 20 mg and works up toward 100 mg, and puts about 300 mg a week subQ as the ceiling without further evaluation. One competitor guide uses the same 300 mg figure.

Sources for these doses

26 sources, each checked by date. Record updated Oct 23, 2026. Numbers are restated in our own words and labelled by where they came from.

Clinical study5

NCT06919328, NR vs NAD+ injectionsClinicalTrials.gov · checked Oct 23, 2026
Grant et al. 2019, IV NAD+ pilotPMC · checked Oct 23, 2026
Narrative review of IV NAD+ (2026)Frontiers in Aging · checked Oct 23, 2026
Reyna et al. 2026, NAD+ vs NR IV tolerabilityFrontiers in Aging · checked Oct 23, 2026

Vendor guide1

NAD+ InjectionEmpower Pharmacy · checked Oct 23, 2026

Competitor guide12

NAD+ dosage chartpeptidedeck.com · checked Oct 23, 2026
NAD+ Quick Startpeptidedosingprotocols.com · checked Oct 23, 2026
MOTS-c vs NADpeptidedeck.com · checked Oct 23, 2026
NAD+ (500 mg vial) dosage protocolpeptidedosages.com · checked Oct 23, 2026

Community-reported8

I have a vial of 500mg NAD (comment: 5 mL, 25 to 50 mg)r/Peptides · checked Oct 23, 2026
Bizarre NAD+ subq reaction (comment: restart low)r/Peptides · checked Oct 23, 2026
NAD+ dosing (comment)r/Peptides · checked Oct 23, 2026
NAD+ dosing (comment)r/Peptides · checked Oct 23, 2026

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