Scalp serum
Who: Community topical practice as summarized by one guide
Timing: Keep minoxidil and vitamin C to a different time of day.
Note: The guide says topical use can run continuously without cycling.
Animal data onlyNot FDA approved
AHK-Cu dosage
Also known as: Copper tripeptide-3 · AHK copper · Alanyl-histidyl-lysine copper · L-Alanyl-L-histidyl-L-lysine-Cu2+ · Ala-His-Lys-Cu
Updated 26 sources
Every AHK-Cu protocol on file, 11 in all, labelled by where each number came from. 1 have titration steps, each step is worked out in U-100 units for a 10 mg vial + 2 mL water.
AHK-Cu dosage by goal
Grouped by goal. Each protocol shows the amount, how often, cycle and rest as the source states them, with every titration step.
Topical, topical5
Hair growth serum
Stronger serum
Note: One review says commercial serums sit around 2 to 5%, with a formulation ceiling near 10%. No approved clinical dose exists.
Community DIY serum
Who: r/Peptides users mixing raw powder into a hyaluronic acid or hair serum
Note: Reported mixes: 1 g per 100 mL of serum for 1%, with one user putting 0.3 mL on the hairline and temples; another uses 0.4% at about 1 mL for the whole head; another says up to 2% is usual.
Copper peptide blend serum
Who: A hair serum sold as following the Anela protocol, reported on the Tressless forum
Note: 1% AHK-Cu alongside 6% GHK-Cu in the same serum. The poster also took 2 to 3 mg of GHK-Cu daily, dermastamped weekly and used ketoconazole shampoo, so results cannot be pinned on the peptide. Another r/tressless user ran a 4% plus 4% blend.
Injected5
Injected titration
Who: One guide's measurement reference. The same page says no validated injectable protocol exists and topical use is the real route.
| When | Dose | U-100 units (10 mg vial + 2 mL water) |
|---|---|---|
| Weeks 1 to 4 | 250 mcg | 5 units · 0.05 mL |
| Weeks 5 to 8 | 350 mcg | 7 units · 0.07 mL |
| Weeks 9 to 12 | 500 mcg | 10 units · 0.1 mL |
Note: From a 10 mg vial in 2 mL (5 mg/mL): 5, 7 and 10 units on a U-100 syringe. The page says this model mirrors the 200 to 500 mcg a topical scalp serum delivers per application.
Injected microgram tiers
Who: Beginner 100 mcg, moderate 250 mcg, aggressive 500 mcg; one guide reports these as community consensus and says the doses are borrowed from GHK-Cu
Note: From a 5 mg vial in 1 mL (5,000 mcg/mL): 2 units for 100 mcg, 5 units for 250 mcg.
Low injected dose
Note: The guide says this comes from informal research and vendor guidance, not a clinical standard.
Research protocol
Note: The page does not name the route; it sits beside the page's injection reconstitution steps. The 12 to 24 week cycle is the page's general figure for hair.
Dosed like GHK-Cu
Who: r/Peptides users who inject it
Note: Users copy the GHK-Cu dose (1 to 2 mg a day, start at 1 mg); one says the sterile version runs 1 to 3 mg or so. One r/tressless user logged 2 mg AHK-Cu plus 2 mg GHK-Cu injections. Others in the same threads say not to inject it at all.
Other, vial (powder)1
Scalp mesotherapy
Who: Practitioner scalp injections, per one calculator site
Note: Injected into the scalp skin, not under it. No study backs a dose.
Doses shown are what sources report, labelled by origin. Not medical advice.
AHK-Cu dosage chart by vial size
Units to draw for the doses sources cite most, at every vial size and water volume.
| Water | Strength | 250 mcgdose, units | 500 mcgdose, units | 1 mgdose, units |
|---|---|---|---|---|
| 5 mg vial | ||||
| 1 mLCompetitor guide | 5 mg/mL | 50.05 mL | 100.1 mL | 200.2 mL |
| 10 mg vial | ||||
| 2 mLCompetitor guide | 5 mg/mL | 50.05 mL | 100.1 mL | 200.2 mL |
| 50 mg vial | ||||
| 3 mLCommunity-reported | 16.667 mg/mL | 1.50.015 mL | 30.03 mL | 60.06 mL |
| 100 mg vial | ||||
| 1 mLCompetitor guide† | 100 mg/mL | 0.30.003 mLunder 1 unit | 0.50.005 mLunder 1 unit | 10.01 mL |
| 2 mLCompetitor guide† | 50 mg/mL | 0.50.005 mLunder 1 unit | 10.01 mL | 20.02 mL |
| 3 mLCommunity-reported† | 33.333 mg/mL | 0.80.008 mLunder 1 unit | 1.50.015 mL | 30.03 mL |
Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.† Water volume sourced for a different vial size. The math is exact either way.Water volumes from
Doses shown are what sources report, labelled by origin. Not medical advice.
Calculate with this peptideYour vial, your water, your dose: AHK-Cu in syringe units, with the math shown.Mixing AHK-Cu
Diluent: Bacteriostatic water
Water volumes sources use: 1 mL, 2 mL, 3 mL
Injection vials hold too little for a scalp serum; topical users buy raw cosmetic powder by the gram instead. Raw powder is not sterile, so it is not for injection or microneedling. One guide warns that a 50 mg vial in 2 mL is too concentrated for microgram doses: 100 mcg would be 0.4 units, too small to measure.
AHK-Cu dosage questions
What is the injected dose?
Published numbers range from 50 mcg to 5 mg, a 100 fold spread: 50 to 200 mcg daily, 100 to 500 mcg daily (reported as community consensus), 250 to 500 mcg titrated over 12 weeks, or 1 to 5 mg two to three times a week. Users who inject usually copy GHK-Cu at 1 to 2 mg a day.
How much water for a vial?
Guides use 2 mL for 10 mg (5 mg/mL) or 1 mL for 5 mg (5 mg/mL). One guide warns that a 50 mg vial is too concentrated for microgram doses; one r/Peptides user mixed a 50 mg vial with 3 mL.
Sources for these doses
14 sources, each checked by date. Record updated Oct 24, 2026. Numbers are restated in our own words and labelled by where they came from.
Competitor guide6
- AHK-Cu reconstitution calculator and referencepeptide-db.com · checked Oct 24, 2026
- AHK-Cu peptide dosage guide, benefits and side effectspeptidemind.com · checked Oct 24, 2026
- AHK-Cu (copper tripeptide) reportoutliyr.com · checked Oct 24, 2026
- AHK-Cu dosage chart, schedule and reconstitution protocolpeptidedosage.org · checked Oct 24, 2026
Community-reported8
- AHK-Cu dosage guide and calculatorpeptideschedule.com, via a competitor guide · checked Oct 24, 2026
- Has anybody tried injectable AHK-Cu for hair? (2026-07-16)r/Peptides · checked Oct 24, 2026
- AHK-cu (2025-10-12)r/Peptides · checked Oct 24, 2026
- GHK-Cu + AHK-Cu experiment (2026-06-02)Tressless Community · checked Oct 24, 2026
Back to the full AHK-Cu page for side effects, storage, stacks and legal status.