Also known as: SS31 · SS 31 · Elamipretide · Elamipretide hydrochloride · MTP-131 · Bendavia · Forzinity
Updated 93 sources
Every SS-31 protocol on file, 35 in all, labelled by where each number came from. 3 have titration steps, each step is worked out in U-100 units for a 10 mg vial + 1 mL water.
Who: Adults and children with Barth syndrome who weigh at least 30 kg, on Forzinity.
Timing: Same time each day. Inject into the belly, at least 2 inches from the navel, or the outer thigh, rotating sites daily.
Note: 40 mg is 0.5 mL of the 80 mg/mL solution. Subcutaneous only, never IV. Skip a missed dose rather than doubling. Community doses sit far below this. No trial tested 1 to 10 mg a day except one 4 mg heart-failure arm.
Who: Barth syndrome patients with eGFR under 30 who are not on dialysis. The label gives no dose for people on dialysis.
Timing: Same time each day. Inject into the belly, at least 2 inches from the navel, or the outer thigh, rotating sites daily.
Who: 30 adults with primary mitochondrial myopathy.
Note: Missed the walk test. Fatigue scores improved nominally, and the gains faded about 2 weeks after stopping.
Who: 218 adults with primary mitochondrial myopathy.
Note: Missed both primary endpoints.
Who: 12 people with Barth syndrome.
Note: The 12-week randomized part missed its primary endpoints. Approval rests on strength gains in the open-label extension.
Who: 102 people with mitochondrial disease caused by nuclear DNA mutations.
Note: The highest daily dose in any trial.
Who: 71 adults with heart failure with reduced ejection fraction. Some guides call this trial PROGRESS-HF.
Note: The 4 mg arm is the only trial dose anywhere near community amounts.
Who: 176 adults with dry age-related macular degeneration (geographic atrophy).
Note: One user runs one 10 mg vial per cycle. Another, age 50, takes 100 mcg a day with TMG and reports more energy. Commonly called pointless on r/Peptides, though some feel helped.
Timing: One user moved it to bedtime because daytime doses made them tired.
Note: 20 units of a 10 mg in 1 mL mix is 2 mg.
Note: 4 mg a day is often called the minimum on r/Peptides. It is a forum rule of thumb, not a trial finding.
| When | Dose | U-100 units (10 mg vial + 1 mL water) |
|---|---|---|
| Days 1 to 20 | 5 mg | 50 units · 0.5 mL |
| Days 21 to 60 | 10 mg | 100 units · 1 mL |
Note: MOTS-c 10 mg 3 times a week joins during the 10 mg phase.
Timing: Morning.
Note: Some users say 1 to 2 mg did little, benefits start at 5 mg, and nothing more comes above that. Another felt no effect after 2 months at 5 mg. One pairs it with NAD+ 100 mg every other day, then moves to MOTS-c.
Note: Offered on r/Peptides as an alternative to 5 mg a day.
Who: One r/Peptides user with chronic fatigue.
Note: Used about 70 mg across varied doses with no benefit before settling on 10 mg a day.
Note: Another user titrated up to 15 mg and reported zero effect after a month.
Timing: Morning.
Note: One user found 20 mg helped where lower doses made them more tired. Another felt jittery on 25 mg, like 2 or 3 energy drinks.
Who: One r/Peptides poster describing a doctor's prescription.
Timing: Morning, or 30 to 60 minutes before a workout.
Timing: Morning, or 30 to 60 minutes before a workout.
Timing: Morning, or 30 to 60 minutes before a workout.
Note: Sensitive starters begin at 0.1 to 1 mg.
Note: The most common range in the guide's community summary.
Timing: Morning.
Note: Performance users go to 10 to 20 mg.
| When | Dose | U-100 units (10 mg vial + 1 mL water) |
|---|---|---|
| Weeks 1 to 2 | 5 mg | 50 units · 0.5 mL |
| Weeks 3 to 8 | 10 mg | 100 units · 1 mL |
Note: The guide is open that trials used 4 or 40 mg; these steps have no trial basis.
| When | Dose | U-100 units (10 mg vial + 1 mL water) |
|---|---|---|
| Weeks 1 to 2 | 5 mg | 50 units · 0.5 mL |
| Weeks 3 to 4 | 10 mg | 100 units · 1 mL |
| Weeks 5 to 8 | 15 mg | 150 units · 1.5 mL(2 draws) |
| Weeks 9 to 12 | 20 mg | 200 units · 2 mL(2 draws) |
Note: No trial basis for these steps.
Timing: Morning, on an empty stomach.
Note: Mostly 5 to 10 mg, sometimes 10 to 20 mg. Cost is the main reason people dose below the trial amount.
Note: Anchored on the 40 mg label dose. The guide notes Barth trials saw no benefit by week 12.
Note: 40 mg is the guide's standard dose.
Note: No trial went above 60 mg a day.
Timing: Morning.
Note: 20 mg is the guide's conservative dose and 40 mg its standard.
Timing: Morning.
Note: The guide's own pages disagree: 0.25 mg/kg in one place, 0.25 to 1.0 mg/kg in another.
Who: 39 older adults in a muscle energy study.
Note: Muscle ATP production rose 27% vs 12% on placebo right after the infusion and was gone by day 7. Research setting only.
Doses shown are what sources report, labelled by origin. Not medical advice.
| Goal | Most reported amounts (mentions) |
|---|---|
| Goal not stated | 5 mg (39), 2 mg (34), 1 mg (26), 10 mg (18) |
| Longevity and energy | 5 mg (11), 1 mg (10), 4 mg (7), 2 mg (6) |
| Injury recovery | 5 mg (4), 4 mg (3), 10 mg (2) |
| Water | Strength | 5 mgdose, units | 10 mgdose, units | 40 mgdose, units |
|---|---|---|---|---|
| 5 mg vial | ||||
| 1 mLCompetitor guide† | 5 mg/mL | 1001 mL | 2002 mL2 draws | 8008 mL8 draws |
| 2 mLCompetitor guide† | 2.5 mg/mL | 2002 mL2 draws | 4004 mL4 draws | 160016 mL16 draws |
| 2.5 mLCompetitor guide† | 2 mg/mL | 2502.5 mL3 draws | 5005 mL5 draws | 200020 mL20 draws |
| 3 mLCompetitor guide† | 1.667 mg/mL | 3003 mL3 draws | 6006 mL6 draws | 240024 mL24 draws |
| 5 mLCompetitor guide† | 1 mg/mL | 5005 mL5 draws | 100010 mL10 draws | 400040 mL40 draws |
| 10 mg vial | ||||
| 1 mLCompetitor guide | 10 mg/mL | 500.5 mL | 1001 mL | 4004 mL4 draws |
| 2 mLCompetitor guide | 5 mg/mL | 1001 mL | 2002 mL2 draws | 8008 mL8 draws |
| 20 mg vial | ||||
| 1 mLCompetitor guide† | 20 mg/mL | 250.25 mL | 500.5 mL | 2002 mL2 draws |
| 2 mLCompetitor guide† | 10 mg/mL | 500.5 mL | 1001 mL | 4004 mL4 draws |
| 2.5 mLCompetitor guide† | 8 mg/mL | 62.50.625 mL | 1251.25 mL2 draws | 5005 mL5 draws |
| 3 mLCompetitor guide† | 6.667 mg/mL | 750.75 mL | 1501.5 mL2 draws | 6006 mL6 draws |
| 5 mLCompetitor guide† | 4 mg/mL | 1251.25 mL2 draws | 2502.5 mL3 draws | 100010 mL10 draws |
| 30 mg vial | ||||
| 1 mLCompetitor guide† | 30 mg/mL | 16.70.167 mL | 33.30.333 mL | 133.31.333 mL2 draws |
| 2 mLCompetitor guide† | 15 mg/mL | 33.30.333 mL | 66.70.667 mL | 266.72.667 mL3 draws |
| 2.5 mLCompetitor guide† | 12 mg/mL | 41.70.417 mL | 83.30.833 mL | 333.33.333 mL4 draws |
| 3 mLCompetitor guide† | 10 mg/mL | 500.5 mL | 1001 mL | 4004 mL4 draws |
| 5 mLCompetitor guide† | 6 mg/mL | 83.30.833 mL | 166.71.667 mL2 draws | 666.76.667 mL7 draws |
| 40 mg vial | ||||
| 2 mLCompetitor guide | 20 mg/mL | 250.25 mL | 500.5 mL | 2002 mL2 draws |
| 50 mg vial | ||||
| 1 mLCompetitor guide | 50 mg/mL | 100.1 mL | 200.2 mL | 800.8 mL |
| 2 mLCompetitor guide | 25 mg/mL | 200.2 mL | 400.4 mL | 1601.6 mL2 draws |
| 2.5 mLCompetitor guide | 20 mg/mL | 250.25 mL | 500.5 mL | 2002 mL2 draws |
| 3 mLCompetitor guide | 16.667 mg/mL | 300.3 mL | 600.6 mL | 2402.4 mL3 draws |
| 5 mLCompetitor guide | 10 mg/mL | 500.5 mL | 1001 mL | 4004 mL4 draws |
Doses shown are what sources report, labelled by origin. Not medical advice.
Calculate with this peptideYour vial, your water, your dose: SS-31 in syringe units, with the math shown.Diluent: Bacteriostatic water
Water volumes sources use: 1 mL, 2 mL, 2.5 mL, 3 mL, 5 mL
0.5 mL in a 10 mg vial gelled or left bits after chilling for some users; most say use at least 1 mL, often 2 to 3, and roll the vial until it dissolves. At 10 to 20 mg per mL a 40 mg dose will not fit in one 1 mL U-100 syringe, so it needs split draws. Approved Forzinity is already a solution and is never mixed. Do not share a syringe with other products; users ask about mixing it with the KLOW blend and nobody has an answer.
The label says skip a missed dose and take the next one at the usual time. Do not double up.
Mostly 40 mg under the skin once a day. NuPower used 60 mg, and one heart-failure trial had a 4 mg arm. IV studies used weight-based infusions: 0.01 to 0.25 mg/kg per hour for 2 hours a day over 5 days in MMPOWER, 0.005 to 0.25 mg/kg per hour over 4 hours in a heart-failure study, and 0.05 mg/kg per hour around heart-attack procedures in EMBRACE.
It is commonly claimed on r/Peptides and has never been tested. One user felt helped at 100 to 500 mcg a day. Others say benefits only start at 5 mg.
There is no data. Guides suggest 4 to 12 weeks on and 2 to 4 off, and one person with mitochondrial disease runs it once every 6 months. Trials and the label dose continuously.
Commonly 1 to 2 mL in a 10 mg vial and 2 to 3 mL in a 50 mg vial. Users who used only 0.5 mL in a 10 mg vial report gelling, so most say use at least 1 mL. At 10 to 20 mg per mL, a 40 mg dose will not fit in one 1 mL syringe.
Guides say 28 days in the fridge. Nobody has tested research vials. The only validated window is 8 days after opening, for the approved solution.
42 sources, each checked by date. Record updated Oct 24, 2026. Numbers are restated in our own words and labelled by where they came from.
Back to the full SS-31 page for side effects, storage, stacks and legal status.