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Thymosin beta-4 dosage

Also known as: Thymosin beta 4 · Tβ4 · TB4 · TB-4 · Full-length thymosin beta-4 · TMSB4X · RGN-259 · RGN-137 · NL005

Updated 26 sources

Every Thymosin beta-4 protocol on file, 10 in all, labelled by where each number came from.

Calculate with Thymosin beta-4Thymosin beta-4 overview

Thymosin beta-4 dosage by goal

Grouped by goal. Each protocol shows the amount, how often, cycle and rest as the source states them, with every titration step.

Intravenous (IV)4

IV safety study (synthetic protein)

Amount
42 to 1,260 mg
How often
One dose, then the same dose daily for 14 days
Route
Intravenous (IV)
Cycle
14 days

Who: 40 healthy volunteers in four cohorts of 10 (2010 Phase 1).

Note: A safety study, not a treatment. IV doses of 42 to 1,260 mg bear no relation to subQ vial doses.

IV single dose (recombinant NL005)

Amount
0.05 to 25 mcg/kg
How often
One dose
Route
Intravenous (IV)

Who: 54 healthy Chinese volunteers in seven ascending cohorts (2021 Phase 1).

IV daily (recombinant NL005)

Amount
0.5 to 5 mcg/kg
How often
Once daily for 10 days
Route
Intravenous (IV)
Cycle
10 days

Who: 30 healthy Chinese volunteers in three cohorts (2021 Phase 1).

IV after a heart attack (recombinant NL005)

Amount
0.5 to 1.5 mcg/kg
How often
First dose 12 hours after PCI, then daily on days 2 to 7
Route
Intravenous (IV)
Cycle
7 days

Who: Adults with acute myocardial infarction after PCI (Phase 2, 90 enrolled).

Note: The registry gives the high dose as 1.0 mcg/kg in the arm and 1.5 mcg/kg in the intervention; the middle dose is 0.5 mcg/kg. Results not posted.

Topical, topical2

Venous stasis ulcer gel (trial)

Amount
0.01 to 0.1%
How often
Once daily on the ulcer
Route
Topical, topical
Cycle
Up to 84 days

Who: Adults with venous stasis ulcers at eight European sites (Phase 2, 73 randomised).

Note: 0.03% looked most promising; about 25% healed completely within 3 months.

Pressure ulcer gel (trial)

Amount
0.01 to 0.1%
How often
Once daily
Route
Topical, topical
Cycle
Up to 84 days

Who: Adults with pressure ulcers (Phase 2, 72 enrolled).

Note: Tested at 0.01, 0.02 and 0.1%.

Injected4

Guide convention (subQ)

Amount
750 mcg
How often
Twice weekly, Monday and Thursday (1,500 mcg a week)
Route
SubQ, vial (powder)

Timing: Morning on dosing days.

Note: The guide says no validated human dose exists. A 5 mg vial lasts about 17 days at this rate.

Off-label loading (borrowed from TB-500)

Amount
2 to 5 mg
How often
Twice weekly
Route
SubQ, vial (powder)
Cycle
4 to 6 weeks, then weekly maintenance

Note: Inherited from TB-500 fragment practice. Not tested in any human trial of the full protein.

Off-label maintenance

Amount
2 to 2.5 mg
How often
Once weekly after loading
Route
SubQ, vial (powder)

Off-label high weekly

Amount
10 mg
How often
Once weekly for 6 weeks, then monthly
Route
SubQ, vial (powder)
Cycle
6 weeks, then monthly

Note: An anecdotal outlier.

Doses shown are what sources report, labelled by origin. Not medical advice.

Thymosin beta-4 dosage chart by vial size

Units to draw for the doses sources cite most, at every vial size and water volume.
Thymosin beta-4: concentration and U-100 syringe units for each vial size and water volume
WaterStrength750 mcgdose, units2 mgdose, units5 mgdose, units
5 mg vial
2 mLCompetitor guide2.5 mg/mL300.3 mL800.8 mL2002 mL2 draws
10 mg vial
2 mLCompetitor guide†5 mg/mL150.15 mL400.4 mL1001 mL
Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.† Water volume sourced for a different vial size. The math is exact either way.Water volumes from

Doses shown are what sources report, labelled by origin. Not medical advice.

Calculate with this peptideYour vial, your water, your dose: Thymosin beta-4 in syringe units, with the math shown.

Mixing Thymosin beta-4

Diluent: Bacteriostatic water

Water volumes sources use: 2 mL

Water volumes for TB-500 vials are not assumed to apply. Check what your vial actually contains before using any TB-500 table.

Thymosin beta-4 dosage questions

What doses did human trials use?

Three very different ranges: 42 to 1,260 mg IV daily for 14 days in a 2010 safety study; 0.5 to 5 mcg per kg IV daily in the newer recombinant programme; and gels of 0.01 to 0.1% or 0.1% eye drops applied to the wound or eye. None were subQ injections.

What subQ dose do people use?

No subQ dose has been tested. One guide uses 750 mcg twice weekly. Off-label protocols borrowed from TB-500 run 2 to 5 mg twice weekly for 4 to 6 weeks, then 2 to 2.5 mg weekly.

How do I mix a 5 mg vial?

One guide adds 2 mL of bacteriostatic water for 2.5 mg/mL, so 750 mcg is 30 units on a U-100 syringe. Use the calculator for other mixes.

How long does mixed thymosin beta-4 last?

Keep it at 2 to 8 C. One guide says 7 to 10 days, another 30 days. Do not freeze it.

Sources for these doses

9 sources, each checked by date. Record updated Oct 24, 2026. Numbers are restated in our own words and labelled by where they came from.

Vendor guide1

  1. TB-500 10 mg product page (sold as full-length thymosin beta-4)Vendor product page · checked Oct 24, 2026

Competitor guide1

  1. Thymosin beta-4 protocol guidepathtopeptides.com · checked Oct 24, 2026

Community-reported1

  1. Thymosin beta-4 report (off-label anecdotal protocols)outliyr.com, via a competitor guide · checked Oct 24, 2026

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