Limited human dataNot FDA approved

AOD-9604 dose

Also known as: AOD9604 · AOD 9604 · Advanced Obesity Drug · AOD-9604 acetate · AOD 9064

Updated Oct 23, 202642 sources

Every AOD-9604 protocol on file, 12 in all, labelled by where each number came from. 1 have titration steps, each step is worked out in U-100 units for a 5 mg vial + 1 mL water.

Weight loss

Calculate with AOD-9604 AOD-9604 overview

AOD-9604 dose by goal

Grouped by goal. Each protocol shows the amount, how often, cycle and rest as the source states them, with every titration step.

Weight loss2

Weight loss, phase 2b oral trial (METAOD006)

Amount: 0.25 to 1 mg
How often: Once daily by mouth; arms took 0.25, 0.5 or 1 mg
Route: Oral
Cycle: 24 weeks

Who: Adults with obesity in a 24-week company trial (536 enrolled, 502 randomized). No significant weight-loss difference from placebo at the 12-week primary endpoint.

Weight loss, phase 2a oral trial (METAOD005)

Amount: 1 to 30 mg
How often: Once daily by mouth; arms took 1, 5, 10, 20 or 30 mg
Route: Oral
Cycle: 12 weeks

Who: About 300 adults with obesity in a 12-week company trial. The 1 mg arm lost 0.22 kg a week against 0.07 kg on placebo, a difference FDA called small.

Fat loss8

Fat loss, most common start (dosing guides and community)

Amount: 250 to 300 mcg
How often: Once daily
Route: Subcutaneous injection
Cycle: 8 to 12 weeks

Who: Adults self-dosing, mostly alongside a GLP-1. No controlled human trial has tested subcutaneous AOD-9604.

Timing: Morning, fasted, water only. Wait 30 to 60 minutes before eating, often doing cardio in that window.

Fat loss, 300 to 500 mcg ramp (dosing guides)

Amount: 300 to 500 mcg
How often: Once daily; 300 mcg for weeks 1 to 4, then 500 mcg to week 12
Route: Subcutaneous injection
Cycle: 12 weeks (8 to 16 discussed)

Who: A dosing-site convention with no data behind the step-up. No controlled human trial has tested subcutaneous AOD-9604.

Timing: Morning, on an empty stomach.

Titration steps for Fat loss, 300 to 500 mcg ramp (dosing guides)
When	Dose	U-100 units (5 mg vial + 1 mL water)
Weeks 1 to 4	300 mcg	6 units · 0.06 mL
Weeks 5 to 12	500 mcg	10 units · 0.1 mL

Fat loss, weight-tiered, under 150 lb (dosing guide)

Amount: 250 to 400 mcg
How often: Once daily
Route: Subcutaneous injection
Cycle: 12 weeks, sometimes 16
Rest: 4 to 6 weeks off

Who: People under 150 lb, per one guide. The weight tiers have no stated basis. No controlled human trial has tested subcutaneous AOD-9604.

Timing: At least 30 minutes before food.

Fat loss, weight-tiered, over 150 lb (dosing guide)

Amount: 300 to 500 mcg
How often: Once daily
Route: Subcutaneous injection
Cycle: 12 weeks, sometimes 16
Rest: 4 to 6 weeks off

Who: People over 150 lb, per one guide. The weight tiers have no stated basis. No controlled human trial has tested subcutaneous AOD-9604.

Timing: At least 30 minutes before food.

Fat loss, split dose (dosing guide)

Amount: 250 mcg
How often: Twice daily, 250 mcg each
Route: Subcutaneous injection
Cycle: 12 weeks

Who: One guide's split schedule. No controlled human trial has tested subcutaneous AOD-9604.

Timing: First dose fasted in the morning, second at bedtime at least 3 hours after food.

Fat loss, community cheat-sheet range

Amount: 250 to 500 mcg
How often: Once daily
Route: Subcutaneous injection
Cycle: 4 to 12 weeks
Rest: 2 to 4 weeks off

Who: From a widely shared r/PeptideGuide cheat sheet. No controlled human trial has tested subcutaneous AOD-9604.

Timing: Fasted.

Fat loss, workout mornings only, with retatrutide (community)

Amount: 300 mcg
How often: 4 times a week, on training mornings
Route: Subcutaneous injection

Who: One r/NTNPerformance poster running AOD on top of retatrutide. No trial tested this.

Timing: Morning of a workout day.

Fat loss, 500 mcg before fasted cardio (community)

Amount: 500 mcg
How often: Once daily
Route: Subcutaneous injection

Who: Self-reported Reddit runs, often stacked with other peptides or retatrutide. No trial tested this.

Timing: Fasted, before morning cardio.

Other protocols: injected2

Recomp, 5 days on and 2 off (community)

Amount: 300 mcg
How often: 5 days a week (5 on, 2 off)
Route: Subcutaneous injection

Who: Self-reported Reddit runs, usually with CJC-1295/ipamorelin at night. One poster ran 5 on, 2 off for 30 days. No trial tested this.

Timing: Morning, fasted.

High-dose outliers (community)

Amount: 1 to 2 mg
How often: Daily, or 5 days on and 2 off
Route: Subcutaneous injection

Who: A few Reddit posters, well above the usual range. Length of run not stated. No trial tested subcutaneous doses at this level.

Doses shown are what sources report, labelled by origin. Not medical advice.

Community-reported amounts

The AOD-9604 amounts people mention most on Reddit, by goal. Counts, not recommendations.

AOD-9604 amounts reported on Reddit, by goal, with mention counts
Goal	Most reported amounts (mentions)
Weight loss	300 mcg (6), 1 mg (2)
Goal not stated	3 mg (2), 5 mg (2), 250 mcg (2), 500 mcg (2)

From 868 Reddit posts and comments, last mined Oct 23, 2026.

AOD-9604 dose chart by vial size

Units to draw for the doses sources cite most, at every vial size and water volume.

AOD-9604: concentration and U-100 syringe units for each vial size and water volume
Water	Strength	250 mcgdose, units	300 mcgdose, units	500 mcgdose, units
2 mg vial
3 mLCompetitor guide	0.667 mg/mL	37.50.375 mL	450.45 mL	750.75 mL
5 mg vial
1 mLCompetitor guide	5 mg/mL	50.05 mL	60.06 mL	100.1 mL
2 mLCompetitor guide	2.5 mg/mL	100.1 mL	120.12 mL	200.2 mL
3 mLCommunity-reported	1.667 mg/mL	150.15 mL	180.18 mL	300.3 mL
10 mg vial
1 mLCompetitor guide^†	10 mg/mL	2.50.025 mL	30.03 mL	50.05 mL
2 mLCompetitor guide^†	5 mg/mL	50.05 mL	60.06 mL	100.1 mL
3 mLCommunity-reported^†	3.333 mg/mL	7.50.075 mL	90.09 mL	150.15 mL

Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.† Water volume sourced for a different vial size. The math is exact either way.Water volumes from

Doses shown are what sources report, labelled by origin. Not medical advice.

Calculate with this peptideYour vial, your water, your dose: AOD-9604 in syringe units, with the math shown.

Mixing AOD-9604

Diluent: Bacteriostatic water, or 0.6% acetic acid for vials that gel

Water volumes sources use: 1 mL, 2 mL, 3 mL

AOD-9604 often gels, clumps or turns cloudy after mixing. FDA links its disulfide bridge to degradation and aggregation. Community fixes: 0.6% acetic acid alone or mixed with bacteriostatic water (one poster used 1 mL acetic acid plus 2 mL water in a 10 mg vial, another 0.5 mL plus 2.5 mL), and rolling instead of shaking. Some still gel with acid. Discard vials that stay cloudy. Clinics sell 600 mcg/mL 10 mL vials, 1 mg capsules and 250 mcg dissolving tablets.

AOD-9604 dose questions

What dose of AOD-9604 is typical?

250 to 300 mcg injected once daily, fasted, is the most common community dose, and some go up to 500 mcg. None of that has been tested in a trial. The human trials used oral doses from 0.25 mg to 30 mg a day.

How much bacteriostatic water do I add to a 5 mg vial?

Dosing guides use 1, 2 or 3 mL. More water makes each dose a bigger, easier-to-measure draw. Use the calculator for the exact syringe units.

How long is an AOD-9604 cycle?

Guides and community cheat sheets quote 8 to 16 weeks on with 2 to 6 weeks off. Neither number comes from data.

Sources for these doses

18 sources, each checked by date. Record updated Oct 23, 2026. Numbers are restated in our own words and labelled by where they came from.

Clinical study2

FDA Evaluation of AOD-9604-Related Bulk Drug Substances (PCAC briefing, 11/5/2024)FDA · checked Oct 23, 2026
Stier, Vos, Kenley 2013, Safety and Tolerability of the Hexadecapeptide AOD9604 in Humans, J Endocrinol Metab 3(1-2):7-15Journal of Endocrinology and Metabolism · checked Oct 23, 2026

Competitor guide5

AOD-9604 dosingpeptideprotocolwiki.com · checked Oct 23, 2026
AOD 9604 dosage and side effectspeptidesexplorer.com · checked Oct 23, 2026
AOD-9604 protocolpeptidedosingprotocols.com · checked Oct 23, 2026
AOD-9604 (5 mg Vial) Dosage Protocolpeptidedosages.com · checked Oct 23, 2026

Community-reported11

Peptide cheat sheet: dosing, reconstitution (AOD 5 mg/3 mL, 250-500 mcg, 4-12 wk, 2-4 off)r/PeptideGuide · checked Oct 23, 2026
Comment: fasted AOD, nothing for 30-60 min, then cardior/Biohacking · checked Oct 23, 2026
Maximizing weight loss/muscle for 8 weeks (AOD 300 mcg AM, no food 30 min)r/Biohackers · checked Oct 23, 2026
2nd peptide cycle looking for feedback (AOD 300 mcg 5x/week + CJC/Ipa)r/Peptidesource · checked Oct 23, 2026

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