Also known as: PE 22-28 · PE22-28 · Mini-spadin · GVSWGLR · Gly-Val-Ser-Trp-Gly-Leu-Arg · PE 22-28 acetate
Updated 21 sources
Every PE-22-28 protocol on file, 8 in all, labelled by where each number came from. 1 have titration steps, each step is worked out in U-100 units for a 10 mg vial + 2 mL water.
Who: Adults self-dosing; no human study exists.
Timing: Morning, because lethargy is the main complaint at higher doses.
Note: Community reports keep the daily total under 600 mcg. Nasal absorption of PE-22-28 has never been measured.
Who: Beginner, intermediate and advanced tiers; no human study exists.
Note: Tiers: 100 to 200 mcg beginner, 200 to 300 intermediate, 300 to 500 advanced, per administration. 1 to 2 weeks at the lowest tier before any increase. The guide says nothing published supports a benefit above 500 mcg a day. Subcutaneous alternative: 100 to 300 mcg.
Note: Speakers quoted on the same page report lethargy at about 800 mcg and good results at 400 mcg; one suggests 400 mcg in each nostril.
Note: A clinic's patient guide; some prescribers cycle 8 weeks on, 4 off. The same range is given for subcutaneous use.
Who: Adults self-dosing; no human study exists.
Note: The injected variant runs lower than nasal. Some users report stronger effects by injection but more lethargy at higher doses.
Who: Adults self-dosing; no human study exists.
Timing: A consistent daily time; rotate sites.
| When | Dose | U-100 units (10 mg vial + 2 mL water) |
|---|---|---|
| Weeks 1 to 2 | 50 mcg | 1 units · 0.01 mL |
| Weeks 3 to 8 | 100 mcg | 2 units · 0.02 mL |
| Weeks 9 to 12 | 150 mcg | 3 units · 0.03 mL |
| Weeks 13 to 16 | 200 mcg | 4 units · 0.04 mL |
Note: The 150 and 200 mcg steps are optional, only if well tolerated. The guide says many find 100 to 150 mcg enough.
Note: Outlier. The page describes PE-22-28 as a spinorphin analogue acting on TRPV1, which contradicts the primary literature, and its daily total of 1,000 mcg is above the 600 mcg ceiling most users keep. It starts at 250 mcg twice daily and moves to 500 mcg twice daily after assessing mood, without saying when.
Doses shown are what sources report, labelled by origin. Not medical advice.
| Water | Strength | 50 mcgdose, units | 100 mcgdose, units | 200 mcgdose, units |
|---|---|---|---|---|
| 8 mg vial | ||||
| 2 mLCompetitor guide† | 4 mg/mL | 1.30.013 mL | 2.50.025 mL | 50.05 mL |
| 3 mLCompetitor guide† | 2.667 mg/mL | 1.90.019 mL | 3.80.038 mL | 7.50.075 mL |
| 5 mLCompetitor guide† | 1.6 mg/mL | 3.10.031 mL | 6.30.063 mL | 12.50.125 mL |
| 10 mg vial | ||||
| 2 mLCompetitor guide | 5 mg/mL | 10.01 mL | 20.02 mL | 40.04 mL |
| 3 mLCompetitor guide | 3.333 mg/mL | 1.50.015 mL | 30.03 mL | 60.06 mL |
| 5 mLCompetitor guide | 2 mg/mL | 2.50.025 mL | 50.05 mL | 100.1 mL |
| 20 mg vial | ||||
| 3 mLCompetitor guide | 6.667 mg/mL | 0.80.008 mLunder 1 unit | 1.50.015 mL | 30.03 mL |
Doses shown are what sources report, labelled by origin. Not medical advice.
Calculate with this peptideYour vial, your water, your dose: PE-22-28 in syringe units, with the math shown.Diluent: Bacteriostatic water
Water volumes sources use: 2 mL, 3 mL, 5 mL
For a nasal sprayer, work backward from the mcg you want per 0.1 mL spray: at 5 mg/mL one spray is 500 mcg, above the usual range, so nasal users add more water. Swirl, do not shake.
Community reports run 200 to 400 mcg by nasal spray, or 100 to 200 mcg by subcutaneous injection, once each morning, with the daily total kept under 600 mcg. None of this comes from a human study.
2 mL is the common choice, giving 5 mg/mL, so 200 mcg is 4 units on a U-100 syringe. One guide uses 3 mL (3.33 mg/mL). For a nasal sprayer at about 0.1 mL per spray, 5 mL gives 200 mcg per spray.
3.0 to 4.0 mcg/kg by intraperitoneal injection, as one dose or once daily for 4 days; 1 mg/kg worked by gavage. A stroke study gave 0.03 mcg/kg daily for 7 days, then 3 mcg/kg on 4 days a week. One wiki says 0.1 mg/kg; the primary paper does not.
Usually 4 to 8 weeks, then a break, in community reports. That habit is borrowed from other peptides; the longest mouse study ran a few weeks.
10 sources, each checked by date. Record updated Oct 24, 2026. Numbers are restated in our own words and labelled by where they came from.
Back to the full PE-22-28 page for side effects, storage, stacks and legal status.