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Limited human dataNot FDA approved

Semax dosage

Also known as: MEHFPGP · Met-Glu-His-Phe-Pro-Gly-Pro · ACTH(4-10) analog · ACTH(4-7)PGP · (Pro8,Gly9,Pro10)ACTH-(4-10) · Semax acetate

Updated 69 sources

Every Semax protocol on file, 30 in all, labelled by where each number came from.

Calculate with SemaxSemax overview

Semax dosage by goal

Grouped by goal. Each protocol shows the amount, how often, cycle and rest as the source states them, with every titration step.

Standard nasal2

Standard nasal (guide)

Amount
300 to 600 mcg
How often
Once daily, before noon
Route
Nasal, nasal spray
Cycle
10 to 20 days on
Rest
2 weeks off

Timing: Before noon, as 1 to 2 drops per nostril

Note: Starts at 300 mcg.

Standard nasal (daily total, guide)

Amount
200 to 600 mcg
How often
Daily total, in 1 or 2 doses
Route
Nasal, nasal spray
Cycle
10 to 30 days
Rest
2 to 4 weeks off

Timing: Morning; take any second dose before 2 to 3 PM

Note: Start with 100 to 200 mcg once daily for 3 to 4 days to check tolerance. A higher tier runs 600 to 900 mcg a day.

Nasal start2

Nasal start (daily total, guide)

Amount
600 mcg
How often
Daily total, split into 2 or 3 doses
Route
Nasal, nasal spray
Cycle
Up to 60 days at 600 mcg a day; up to 30 days above 900 mcg a day

Nasal start (guide)

Amount
150 mcg
How often
Twice daily, morning and noon
Route
Nasal, nasal spray
Cycle
3 to 6 weeks on, then the same off

Note: The same guide puts the nasal ceiling at 750 to 1,000 mcg a day.

Subcutaneous2

Subcutaneous (guide)

Amount
100 to 500 mcg
How often
1 to 2 times a day
Route
SubQ, vial (powder)
Cycle
10 to 14 days
Rest
1 to 2 weeks washout

Subcutaneous (daily total, guide)

Amount
100 to 300 mcg
How often
Daily total
Route
SubQ, vial (powder)
Cycle
3 to 6 weeks on, then the same off

Timing: Morning

Note: Suggests starting at 250 mcg.

Other protocols: nasal, nasal spray18

Mental fatigue and adaptation, Russian label (0.1%)

Amount
200 to 300 mcg
How often
2 to 3 times in the first half of the day (400 to 900 mcg a day)
Route
Nasal, nasal spray
Cycle
3 to 5 days, repeated if needed

Who: Adults; 2 to 3 drops of 0.1% drops in each nostril per dose

Note: The label puts one 0.1% drop at 50 mcg; the per-dose mcg is one guide's translation of the label.

Memory and attention with vascular brain disease, Russian label (0.1%)

Amount
200 to 2,000 mcg
How often
4 times a day (800 to 8,000 mcg a day)
Route
Nasal, nasal spray
Cycle
10 to 14 days

Who: Adults with memory or attention problems from vascular brain disease

Note: Single doses of 3 to 30 mcg/kg. The label caps each round at 2 to 3 drops per nostril; bigger doses are given as repeat rounds 10 to 15 minutes apart.

After brain injury, neurosurgery or anesthesia, Russian label (0.1%)

Amount
1,400 to 3,500 mcg
How often
3 times a day
Route
Nasal, nasal spray
Cycle
3 to 5 days, up to 14 days

Who: Adults recovering from traumatic brain injury, brain surgery or anesthesia

Note: 40 to 50 mcg/kg per dose.

Optic nerve disease, Russian label (0.1%, daily total)

Amount
600 to 900 mcg
How often
Daily total
Route
Nasal, nasal spray
Cycle
10 days

Who: Adults with optic nerve disease

Children 7 and older, Russian label (0.1%, daily total)

Amount
200 to 400 mcg
How often
Daily total, given as 1 to 2 drops per nostril twice a day, morning and afternoon
Route
Nasal, nasal spray
Cycle
30 days

Who: Children 7 and older with minimal brain dysfunction, including ADHD

Moderate ischemic stroke, Russian label (1%)

Amount
2,000 to 3,000 mcg
How often
3 to 4 times a day, 3 to 4 hours apart (6,000 to 12,000 mcg a day)
Route
Nasal, nasal spray
Cycle
10 days

Who: Adults with moderate ischemic stroke, under medical care

Note: Uses the 1% drops, where one drop is 500 mcg.

Severe ischemic stroke, Russian label (1%)

Amount
3,000 to 4,000 mcg
How often
4 to 5 times a day (12,000 to 20,000 mcg a day)
Route
Nasal, nasal spray
Cycle
10 days

Who: Adults with severe ischemic stroke, under medical care

Note: Uses the 1% drops, where one drop is 500 mcg.

Acute ischemic stroke trial (daily total)

Amount
12 to 18 mg
How often
Daily total
Route
Nasal, nasal spray
Cycle
5 or 10 day courses

Who: 30 treated stroke patients vs 80 controls (Gusev 1997)

Note: 12 mg a day worked best in moderate stroke and 18 mg in severe stroke.

Post-stroke trial (daily total)

Amount
6,000 mcg
How often
Daily total
Route
Nasal, nasal spray
Cycle
10 days, two courses
Rest
20 days between the two courses

Who: 110 post-stroke patients (Gusev 2018)

Weight-based dose (developer review)

Amount
15 to 50 mcg/kg
How often
Once daily; effects reported to last 20 to 24 hours
Route
Nasal, nasal spray

Note: From a review by the developers. No fixed schedule is given.

Nootropic, once daily (guide)

Amount
200 to 600 mcg
How often
Once daily
Route
Nasal, nasal spray
Cycle
2 to 4 weeks

Timing: Avoid dosing near bedtime

Nootropic nasal (daily total, guide)

Amount
600 to 1,500 mcg
How often
Daily total, split into 2 doses
Route
Nasal, nasal spray
Cycle
10 to 14 days for a short course, or 4 to 6 weeks on
Rest
2 to 4 weeks off after a longer run

Timing: Morning and early afternoon

High-performance nasal (guide)

Amount
600 to 900 mcg
How often
Daily, before noon
Route
Nasal, nasal spray
Cycle
10 to 20 days on
Rest
2 weeks off

Note: The same guide gives 600 mcg nasal for neuroprotection goals.

Nasal, 0.1% solution (daily total, guide)

Amount
200 to 600 mcg
How often
Daily total, split into 2 or 3 doses
Route
Nasal, nasal spray
Cycle
10 to 14 days
Rest
1 to 2 weeks washout

General nootropic (guide)

Amount
200 to 600 mcg
How often
1 to 2 times a day, 3 to 4 hours apart
Route
Nasal, nasal spray
Cycle
10 to 14 days on
Rest
An equal period off

Timing: Morning

Cognitive impairment (guide)

Amount
600 to 900 mcg
How often
2 to 3 times a day
Route
Nasal, nasal spray

Stroke recovery (guide)

Amount
1,200 to 1,800 mcg
How often
3 times a day
Route
Nasal, nasal spray
Cycle
5 to 14 days in the acute phase

Daily nasal (community)

Amount
200 to 600 mcg
How often
Once daily, usually in the morning
Route
Nasal, nasal spray

Note: Most start around 200 to 250 mcg; 300 to 500 mcg gets called the sweet spot. Some insist on 3 doses a day because Semax clears fast, but most dose once in the morning. One commenter argues for label-scale dosing: 15 to 50 mcg/kg works out to 1,050 to 3,500 mcg a day at 70 kg.

Other protocols: injected6

Subcutaneous planning dose (guide)

Amount
500 mcg
How often
Once daily
Route
SubQ, vial (powder)

Note: The FDA found no human safety data for injected Semax.

Neuroprotection, subcutaneous (guide)

Amount
100 to 200 mcg
How often
Daily
Route
SubQ, vial (powder)

Injectable (guide)

Amount
500 to 1,000 mcg
How often
Once daily
Route
SubQ, vial (powder)
Cycle
4 to 8 weeks

Daily subcutaneous (community)

Amount
500 to 1,000 mcg
How often
Once daily, in the morning
Route
SubQ, vial (powder)
Cycle
4 weeks on
Rest
4 weeks off

Note: Reports include 500 mcg every morning, 0.6 mg daily, and 500 mcg rising to 1 mg. One user starts with a small test dose, then 500, then 800 mcg.

Weekday subcutaneous (community)

Amount
1 mg
How often
Once each morning, 5 days on and 2 off
Route
SubQ, vial (powder)
Cycle
1 month

High-dose subcutaneous block (community)

Amount
1,500 mcg
How often
Once daily
Route
SubQ, vial (powder)
Cycle
4 weeks, repeated every 10 weeks

Doses shown are what sources report, labelled by origin. Not medical advice.

Community-reported amounts

The Semax amounts people mention most on Reddit, by goal. Counts, not recommendations.
Semax amounts reported on Reddit, by goal, with mention counts
GoalMost reported amounts (mentions)
Focus and mood500 mcg (16), 200 mcg (8), 300 mcg (7), 100 mcg (6)
Goal not stated10 mg (10), 1 mg (8), 200 mcg (8), 500 mcg (8)
From 900 Reddit posts and comments, last mined Oct 23, 2026.

Semax dosage chart by vial size

Units to draw for the doses sources cite most, at every vial size and water volume.
Semax: concentration and U-100 syringe units for each vial size and water volume
WaterStrength100 mcgdose, units500 mcgdose, units1,000 mcgdose, units
5 mg vial
2 mLCommunity-reported2.5 mg/mL40.04 mL200.2 mL400.4 mL
2.5 mLCompetitor guide2 mg/mL50.05 mL250.25 mL500.5 mL
10 mg vial
3 mLCommunity-reported3.333 mg/mL30.03 mL150.15 mL300.3 mL
20 mg vial
2 mLCompetitor guide10 mg/mL10.01 mL50.05 mL100.1 mL
4 mLCompetitor guide5 mg/mL20.02 mL100.1 mL200.2 mL
5 mLCompetitor guide4 mg/mL2.50.025 mL12.50.125 mL250.25 mL
30 mg vial
2 mLCommunity-reported†15 mg/mL0.70.007 mLunder 1 unit3.30.033 mL6.70.067 mL
2.5 mLCompetitor guide†12 mg/mL0.80.008 mLunder 1 unit4.20.042 mL8.30.083 mL
3 mLCommunity-reported†10 mg/mL10.01 mL50.05 mL100.1 mL
4 mLCompetitor guide†7.5 mg/mL1.30.013 mL6.70.067 mL13.30.133 mL
5 mLCompetitor guide†6 mg/mL1.70.017 mL8.30.083 mL16.70.167 mL
Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.† Water volume sourced for a different vial size. The math is exact either way.Water volumes from

Doses shown are what sources report, labelled by origin. Not medical advice.

Calculate with this peptideYour vial, your water, your dose: Semax in syringe units, with the math shown.

Mixing Semax

Diluent: Bacteriostatic water for injection; sterile saline to dilute nasal sprays

Water volumes sources use: 2 mL, 2.5 mL, 3 mL, 4 mL, 5 mL

For 20 mg vials, 4 mL is the default in one guide. For sprays, one guide dissolves 10 mg in 3 mL and tops up with sterile saline to 5 mL (200 mcg per 0.1 mL) or 10 mL (100 mcg per 0.1 mL).

Semax dosage questions

How much Semax per day?

The Russian label uses 400 to 900 mcg a day for mental fatigue, split into 2 to 3 doses before midday. Its stroke doses run 6,000 to 20,000 mcg a day. On r/Peptides, 200 to 600 mcg a day is typical, and most guides cluster at 200 to 600 mcg for focus.

How many mcg per drop or spray?

Per the label, one drop of 0.1% is 50 mcg and one drop of 1% is 500 mcg. One guide says about 30 mcg per 0.1% drop. Premixed research sprays at 30 mg per 10 mL give about 300 mcg per pump. Pumps deliver about 0.1 to 0.13 mL per press by user reports, and 0.025 to 0.20 mL across devices, so count presses on a known volume to calibrate.

How do I make a nasal spray from a 10 mg vial?

One guide dissolves 10 mg in 3 mL, then tops up with sterile saline to 5 mL for 200 mcg per 0.1 mL spray, or to 10 mL for 100 mcg per spray. One user mixes to 250 mcg per spray. Calibrate your pump before trusting the math.

How long should a cycle be?

Label courses run 3 to 14 days and can be repeated. Users run 4 weeks on and 4 off, a month on and a month off, or the first ten days of every month. Guides range from 10 to 14 days up to 4 to 6 weeks, with breaks about as long.

Sources for these doses

29 sources, each checked by date. Record updated Oct 23, 2026. Numbers are restated in our own words and labelled by where they came from.

Clinical study3

  1. Semax in acute ischemic stroke (Gusev 1997)PubMed · checked Oct 23, 2026
  2. Semax at different stages of ischemic stroke (Gusev 2018)PubMed · checked Oct 23, 2026
  3. 15 years of Semax (Ashmarin 1997)PubMed · checked Oct 23, 2026

Regulatory3

  1. Semax instruction for use (English translation of the Russian label)Peptogen (label translation) · checked Oct 23, 2026
  2. Semax 10 mg vial dosage protocolpeptidedosages.com, via a competitor guide · checked Oct 23, 2026
  3. FDA briefing document: Semax-related bulk drug substances, PCAC July 23-24 2026FDA · checked Oct 23, 2026

Competitor guide9

  1. Semaxpeptiq.io · checked Oct 23, 2026
  2. Semax quick startpeptidedosingprotocols.com · checked Oct 23, 2026
  3. Semaxpeptidesexplorer.com · checked Oct 23, 2026
  4. Semax dosagepeptides.org · checked Oct 23, 2026

Community-reported14

  1. r/Peptides comment on Semaxr/Peptides · checked Oct 23, 2026
  2. r/Peptides comment on Semaxr/Peptides · checked Oct 23, 2026
  3. Research of effects for selank and semax for 25 yr oldr/Peptides · checked Oct 23, 2026
  4. Interested in SEMAX and SELANK blendsr/Peptides · checked Oct 23, 2026

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