DSIP

DSIP, or delta sleep-inducing peptide, is a chain of 9 amino acids first isolated in 1977 from the blood of rabbits kept asleep by brain stimulation. It was named for its effect on delta, or slow-wave, sleep. Decades later no gene, precursor or receptor has been found, so how it works is still an open question.

Most guides and community posts land on 100 to 300 mcg subQ at night, usually starting at 100 mcg. Outliers run from 25 mcg to 1 mg, and one r/Peptides user tried 2.5 mg. None of these subQ doses has been tested in a trial; every human study used IV dosing at about 21 mcg per kg.

Most guides say 30 to 60 minutes. Others say 1 to 3 hours. The one trial that tracked timing found a slight arousing effect in the first hour and sleep promotion only from the second hour, which argues for the earlier end. Users who felt wired at bedtime often fixed it by dosing earlier.

The evidence is mixed and old. The positive human sleep results come mostly from one research group in 1981 to 1987. Two independent double-blind trials found effects of little clinical significance. FDA's 2026 review called the insomnia evidence inconclusive and at best preliminary. On r/Peptides, reports split between big improvements and nothing at all.

Community reports center on morning grogginess, vivid dreams and headache, plus feeling wired at bedtime for some. In the 1984 IV withdrawal trial, 9 of 107 patients had brief sweating, headache, nausea or vertigo, and 2 had low blood pressure at the first injection. FDA says there is no safety data at all for the subQ route people use.

Guides commonly add 2.5 mL of bacteriostatic water, giving 2 mg per mL, so 100 mcg is 5 units on a U-100 syringe. Another option is 3 mL, where 1 unit is about 16.7 mcg. Use the calculator for your exact vial and dose.

No tolerance study exists. Advice runs from no cycling at all to 10 days on and 10 off, or 2 to 4 weeks on and 2 off. Aggregated community notes mention rebound insomnia after daily use beyond 2 to 3 weeks, and many r/Peptides users run 5 on and 2 off.

No. It has never been approved for any use. In July 2026 an FDA advisory panel voted against letting pharmacies compound it, by a reported 6 to 7, the only one of seven peptides rejected that day. The vote is advisory, and FDA has not made a final decision.

Guides say WADA does not name it on the Prohibited List. Unapproved substances can still fall under WADA's catch-all S0 class, and we could not confirm its status directly with WADA. Tested athletes should treat it as unconfirmed.

Sprays are sold, and FDA found them on US sites. One premixed spray gives 20 mcg per spray, so 20 to 40 mcg a night, compared with about 1.5 mg by IV in the trials. Guides say nasal absorption varies, and no human nasal data exists. One r/Peptides user had nightmares on the nasal form and did better on subQ.

Guides give anything from 24 hours to about 28 days, and none has a stability study behind it. Keep it at 2 to 8 C, away from light, and throw it out if it turns cloudy.

Guides say yes and suggest low-dose melatonin, 0.3 to 0.5 mg, 60 to 90 minutes before bed. The idea is that melatonin handles timing and DSIP handles depth.

No study has measured dependence. r/Peptides users ask often, and a few describe the effect fading or feeling flat. In a 1987 trial, the effect carried into the first placebo night instead of causing rebound.

Guides say it is not a sedative and you can still be woken. Insomnia trials found no daytime sedation.

Nobody knows. FDA puts DSIP at about 8 minutes in human plasma. Guides quote anything from 7 to 30 minutes, and describe it as a trigger that starts a process rather than a drug that has to stay in the blood. No study explains it.

Most it-worked and it-did-nothing posts rely on consumer wearables, and r/Peptides replies point out those stage sleep poorly compared with a sleep lab. One user saw deep sleep crowded into the first half of the night. Treat tracker swings as a hint, not proof.