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Animal data onlyNot FDA approved

5-Amino-1MQ

Also known as: 5 amino 1mq · 5-Amino-1-methylquinolinium · 5-Amino-1-methylquinolinium iodide · 5A1MQ · 5-AMQ · 5-Am-1MQ · Amino 1MQ

Updated 40 sources

5-Amino-1MQ is a small synthetic molecule, not a peptide, that blocks the enzyme NNMT. It is sold beside peptides, mostly as 50 mg oral capsules and sometimes as injectable vials. In obese mice it cut body weight and fat without changing food intake. No human trial exists, so every human dose you see is community or guide practice, and oral and injected doses sit on very different scales.
Calculate with 5-Amino-1MQDosage chart

Quick facts

Half-life
  • About 3.8 hours after IV and 6.9 hours after oral dosing in rats, with 38.4% oral bioavailability. No human data.
Routes
  • Oral
  • Subcutaneous injection
Reported dose ranges
  • 50 to 100 mg (oral)
  • 2.5 to 50 mg (subcutaneous injection)
Vial sizes
  • 5 mg, 10 mg, 50 mg
FDA status
  • Not FDA approved
WADA
  • Prohibited

What people use 5-Amino-1MQ for

  • Fat loss, most often added to a GLP-1 such as retatrutide or tirzepatide to push past a plateau
  • Energy and endurance, as part of a mitochondrial stack with MOTS-c, NAD+, SS-31 or SLU-PP-332
  • Preserving muscle with age, based on aged-mouse muscle injury studies
  • Insulin sensitivity, based on better glucose tolerance in mice and scattered user reports

How it is thought to work. NNMT spends the methyl donor SAM to tag nicotinamide (vitamin B3) for clearance. 5-Amino-1MQ blocks that enzyme. In fat cells this lowered the product 1-MNA, raised NAD+ and SAM inside the cell, and held back fat building. Mice with NNMT knocked down genetically resisted diet-induced obesity. None of this has been measured in people.

5-Amino-1MQ dosing

8 protocols from studies, guides and community reports, grouped by goal. Tap a source badge to see where a number came from.

Oral5

Oral, standard start (competitor guides)

Amount
50 mg
How often
Once daily
Route
Oral
Cycle
8 to 12 weeks on
Rest
4 weeks off
Timing and details

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Oral, standard dose (community)

Amount
50 mg
How often
Once daily
Route
Oral
Timing and details

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Oral, higher dose (competitor guides)

Amount
50 mg
How often
50 mg twice daily (100 mg a day); guides go up to 150 mg a day
Route
Oral
Cycle
8 to 12 weeks on
Rest
4 to 6 weeks off
Timing and details

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Oral, higher dose (community)

Amount
50 mg
How often
Two 50 mg capsules a day (100 mg); some go to 150 mg a day
Route
Oral
Cycle
10 to 12 weeks
Timing and details

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Oral, every other day (community)

Amount
100 mg
How often
100 mg every other day
Route
Oral
Timing and details

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Subcutaneous3

Subcutaneous, guide default (competitor guides)

Amount
2.5 to 5 mg
How often
2.5 mg daily on days 1 and 2, then 5 mg daily (or 2.5 mg twice daily)
Route
Subcutaneous injection
Cycle
About 10 to 20 days per 50 mg vial; no set cycle
Timing and details

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Subcutaneous, common start (community)

Amount
2.5 to 5 mg
How often
Once daily, often 5 days on and 2 off
Route
Subcutaneous injection
Cycle
About 60 days, then a break
Timing and details

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Subcutaneous, high dose (community)

Amount
10 to 50 mg
How often
Once daily at 10, 25 or 50 mg
Route
Subcutaneous injection
Cycle
4 to 6 weeks
Timing and details

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Doses shown are what sources report, labelled by origin. Not medical advice.

Full 5-Amino-1MQ dosage guideEvery titration step in syringe units, missed doses and how to mix.

Vial strengths and syringe units

Every 5-Amino-1MQ vial size with the water volumes sources use. Units are what to draw on a U-100 insulin syringe, worked out exactly.
5-Amino-1MQ: concentration and U-100 syringe units for each vial size and water volume
WaterStrength2.5 mgdose, units5 mgdose, units10 mgdose, units
5 mg vial
1 mLCompetitor guide†5 mg/mL500.5 mL1001 mL2002 mL2 draws
3 mLCompetitor guide†1.667 mg/mL1501.5 mL2 draws3003 mL3 draws6006 mL6 draws
4 mLCompetitor guide†1.25 mg/mL2002 mL2 draws4004 mL4 draws8008 mL8 draws
10 mg vial
1 mLCompetitor guide10 mg/mL250.25 mL500.5 mL1001 mL
3 mLCommunity-reported3.333 mg/mL750.75 mL1501.5 mL2 draws3003 mL3 draws
50 mg vial
3 mLCompetitor guide16.667 mg/mL150.15 mL300.3 mL600.6 mL
4 mLCompetitor guide12.5 mg/mL200.2 mL400.4 mL800.8 mL
Units are on a U-100 insulin syringe (100 units = 1 mL). U-50 and U-30 barrels read the same units.† Water volume sourced for a different vial size. The math is exact either way.Water volumes from

Doses shown are what sources report, labelled by origin. Not medical advice.

Calculate with this peptideYour vial, your water, your dose: 5-Amino-1MQ in syringe units, with the math shown.

What people report

What 5-Amino-1MQ users write on Reddit, counted post by post. These are reports, not measured rates or advice.

Community-reportedFrom 756 Reddit posts and comments, last mined Oct 23, 2026r/Retatrutide, r/Peptides, r/Peptidesource, r/Biohackers

Doses people report for 5-Amino-1MQ

Goal not stated78 mentions

Amounts reported for goal not stated, with mention counts
ReportedMentions
50 mg19
500 mcg14
5 mg9
10 mg9
1 mg7

Weight loss16 mentions

Amounts reported for weight loss, with mention counts
ReportedMentions
5 mg4
50 mg3
84 mg2

Longevity and energy4 mentions

Amounts reported for longevity and energy, with mention counts
ReportedMentions
5 mg2

How often

Dosing frequencies reported, with mention counts
ReportedMentions
Once a day43
5 days on, 2 off5
Twice a week4
Every other day3
Twice a day2

Most mentioned alongside

Side effects people mention

  • Nausea4 mentions

  • Fatigue4 mentions

  • Increased energy3 mentions

Doses shown are what sources report, labelled by origin. Not medical advice.

5-Amino-1MQ side effects

What trials and labels report, kept apart from what users and guides report. Frequencies are worded as each source states them.

Reported in studies and labels1

From clinical trials and regulatory documents.

  • No adverse effects seen in mice

    None observed in the 11-day mouse obesity study, and food intake did not change. In a 28-day mouse study liver enzymes normalized. No human safety data exist.

Reported by users and guides6

From community reports and dosing guides. Not measured rates.

  • Alertness and trouble sleeping

    One community report of staying up late after an evening dose. A competitor guide also lists sleep trouble.

    How people manage it

    • Take it in the morning instead of the evening or before an evening workout.

  • Fatigue

    One community report of tiredness when dosed on consecutive days.

    How people manage it

    • The poster dropped to 3 days a week instead of daily dosing.

  • Frequent urination

    One community report at 2.5 mg a day subcutaneous.

  • Yeast-flavored burps

    One community report on oral dosing.

  • Sweating at high doses

    A single unsupported claim in one community comment.

  • Mild stomach upset and loose stools

    Listed by a competitor guide for the first week. Not sourced to any study.

All 11 5-Amino-1MQ side effects and how people manage themEvery management tip, who should not use it, and side-effect questions.

Storage

Powder (before mixing)

Temperature
-20 to 8 °C

Powder vials: freezer at -20 °C for long-term storage (one guide says up to 24 months), or 2 to 8 °C for the short term. Capsules: room temperature, which one guide says lasts up to 12 months without citing a source. One community user keeps bulk powder frozen.

Mixed vial

Temperature
2 to 8 °C
Use within
14 days

Keep the mixed vial at 2 to 8 °C, away from light, and use it within 2 to 4 weeks. Do not refreeze. No manufacturer in-use stability data was found.

Mixed with: Bacteriostatic water

Mixing. These sizes are injectable powder vials. Most people take 5-Amino-1MQ as oral capsules, where 50 mg per capsule is the common unit and no mixing is needed. A prefilled 50 mg pen is also reported in community posts. Guides mix the 50 mg vial with 3 to 4 mL. No water volume was found for the 5 mg vial. Oral and injected doses are on different scales, so never convert one to the other.

Stacks people report

Combinations from guides and community posts, with the reason given.
  • Retatrutide

    The GLP-1 cuts appetite and 5-Amino-1MQ is meant to help burn what gets released. Users add it on a plateau or run it from the start with 50 mg capsules twice daily. No human interaction data exist.

  • Tirzepatide

    Added to push past a weight-loss stall on tirzepatide. Same logic as with retatrutide: appetite from the GLP-1, cellular energy use from 5-Amino-1MQ. No interaction study exists.

  • NAD+

    Blocking NNMT stops some nicotinamide from being cleared, which in theory supports NAD+. One user takes it about 30 minutes after an NAD+ injection. Some posters worry about methylation load with this pair.

  • MOTS-c

    A mitochondrial energy stack. A common pattern is MOTS-c 2 to 5 mg a few times a week alongside 5 mg of 5-Amino-1MQ.

  • SS-31

    Grouped with MOTS-c and 5-Amino-1MQ in community mitochondrial stacks aimed at energy and endurance.

  • SLU-PP-332

    An exercise-mimetic pairing for endurance. One user ran 3 mg subcutaneous with SLU-PP-332 500 mcg oral, 5 days on and 2 off, for about 60 days. Another called the pair redundant.

  • AOD-9604

    Combined in fat-loss blends, sometimes with 5-Amino-1MQ at around 400 mcg per injection.

  • Tesamorelin

    Paired in fat-loss protocols by a competitor guide.

Compare 5-Amino-1MQ

Evidence and legal status

Evidence: animal data only

Cell studies, an 11-day mouse obesity study (20 mg/kg three times daily, about 5% weight loss), a 28-day mouse obesity and liver study, an aged-mouse muscle study (5 and 10 mg/kg a day, peak torque up about 70%) and a rat PK study. ClinicalTrials.gov lists zero human studies. Most papers come from one lab group, and the 2024 paper discloses commercial ties.

FDA and WADA

Not FDA approved

No approval, no trial program and not on FDA's compounding lists.

Not FDA approved for any use, and no human trials are registered. It is not on the 503A bulks list or in any 503A nominated-substance category, and FDA told an outsourcing facility in January 2026 that it cannot be compounded under 503B either.

WADA does not name 5-Amino-1MQ on its 2026 List. Section S0 bans at all times any pharmacological substance with no human-therapeutic approval from any government health authority, and on our reading that covers it. The related MOTS-c and BAM15 are named under S4.4.1. It is also absent from FDA's significant-safety-risks page, and whether anyone ever nominated it for 503A was not found.

WADA: Prohibited

5-Amino-1MQ questions

Is 5-Amino-1MQ a peptide?

No. It is a small synthetic molecule, a methylquinolinium salt usually sold as the iodide, that blocks the enzyme NNMT. It gets sold and searched as a peptide because it sits on the same shelves.

What is the usual oral dose of 5-Amino-1MQ?

Guides and community posts start at 50 mg a day, usually one capsule in the morning. Many step up to 100 mg a day split into two doses, and some go to 150 mg. No human trial has tested any of these.

What is the injection dose of 5-Amino-1MQ?

Dosing guides start at 2.5 mg a day and move to 5 mg a day. Reddit reports range from 0.4 mg in blends up to 50 mg a day, and one user summed up the spread as anywhere from 100 mcg to 100 mg. A claim that 12.5 mg saturates NNMT in humans has no data behind it.

Should I take 5-Amino-1MQ orally or by injection?

In rats, about 38% of an oral dose was absorbed and the oral half-life was longer than after an IV dose. Community oral doses run 10 to 50 times the injected ones, and users split on which works better. Some argue a small molecule makes more sense by mouth. No human data settle it, and the two dose scales should never be converted one to the other.

How much water goes in a 50 mg vial of 5-Amino-1MQ?

Dosing guides use 3 to 4 mL of bacteriostatic water for the 50 mg vial. Enter your vial and water volume in the calculator to get the exact draw for your dose.

What are the side effects of 5-Amino-1MQ?

There are no human safety data. Mice showed no observable adverse effects. Community reports include poor sleep when taken late, fatigue on consecutive days and frequent urination. Competitor guides list mild stomach upset, headache, warmth and a faster resting heart rate without citing studies.

Does 5-Amino-1MQ work? What results do people see?

The animal results are real: obese mice lost about 5% of body weight in 11 days without eating less, and aged mice regrew injured muscle better. Human reports are mixed, and many users say they noticed nothing at all.

How long is a 5-Amino-1MQ cycle?

Guides quote 8 to 12 weeks on and 4 to 6 weeks off. No study supports any particular cycle length.

Can I take 5-Amino-1MQ with semaglutide, tirzepatide or retatrutide?

It is commonly stacked with GLP-1 drugs, usually to break a weight-loss plateau. No study has looked at how they interact.

Does 5-Amino-1MQ raise NAD+?

It did inside fat cells in a lab study, along with SAM. Nobody has measured it in humans.

Is 5-Amino-1MQ FDA approved or legal to compound?

No. It has no FDA approval and no registered human trials. It is not on the 503A bulks list or in any 503A category, and in January 2026 FDA warned an outsourcing facility that it is ineligible for 503B compounding.

Is 5-Amino-1MQ banned in sport?

WADA does not name it, but section S0 bans any substance with no human-therapeutic approval anywhere. On our reading that covers 5-Amino-1MQ, so tested athletes should treat it as prohibited.

What is the half-life of 5-Amino-1MQ?

In rats it was about 3.8 hours after an IV dose and 6.9 hours after an oral dose. No human half-life has been measured.

Ask about 5-Amino-1MQ

Answers come from this site's pages only, with a link to each source.

Sources

40 sources, each checked by date. Record updated Oct 23, 2026. Numbers are restated in our own words and labelled by where they came from.

Competitor guide3

  1. 5-Amino-1MQ (50 mg Vial) Dosage Protocolpeptidedosages.com · checked Oct 23, 2026
  2. 5-Amino-1MQ protocolpeptidedosingprotocols.com · checked Oct 23, 2026
  3. 5-Amino-1MQpeptidesexplorer.com · checked Oct 23, 2026

Community-reported27

  1. Comment: 10 mg vial mixed with 3 mL bacteriostatic water, asking for a starting doseReddit r/PeptideGuide · checked Oct 23, 2026
  2. Comment: 50 mg oral each morning plus MOTS-c, insulin resistance (type 1 diabetes)Reddit r/diabetes_t1 · checked Oct 23, 2026
  3. Comment: 2 x 50 mg oral daily for 10 to 12 weeks with retatrutide and MOTS-cReddit r/Biohacking · checked Oct 23, 2026